Document Detail


Deleterious effects of endotoxin on cultured endothelial cells: an in vitro model of vascular injury.
MedLine Citation:
PMID:  6268539     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of endotoxin-triggered granulocytes on the viability of endothelial cells in vitro was investigated. Endotoxin or its lipid A component caused granulocytes to adhere to and significantly damage cultured endothelial cells. Fresh serum is not necessary but does amplify both adherence and endothelial injury. Much of the endothelial injury was inhibited by free-radical scavengers or by blocking granulocyte adhesion to endothelial cells and appears to result from free radical production by the stimulated granulocyte. Studies in this model suggest a pathogenic role for the endotoxin-triggered granulocyte in the Shwartzman reaction and perhaps related clinical disorders.
Authors:
O Yamada; C F Moldow; T Sacks; P R Craddock; M A Boogaerts; H S Jacob
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Inflammation     Volume:  5     ISSN:  0360-3997     ISO Abbreviation:  Inflammation     Publication Date:  1981 Jun 
Date Detail:
Created Date:  1981-11-24     Completed Date:  1981-11-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7600105     Medline TA:  Inflammation     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  115-26     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacterial Toxins / pharmacology*
Cells, Cultured
Chromium Radioisotopes / diagnostic use
Endothelium / drug effects*,  metabolism
Escherichia coli*
Humans
Hydrogen Peroxide / metabolism,  pharmacology
Lipid A / pharmacology
Lipopolysaccharides / pharmacology*
Neutrophils / drug effects,  metabolism
Superoxides / metabolism,  pharmacology
Umbilical Veins
Grant Support
ID/Acronym/Agency:
AM 15730/AM/NIADDK NIH HHS; CA 13722/CA/NCI NIH HHS; HL 18725/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Toxins; 0/Chromium Radioisotopes; 0/Lipid A; 0/Lipopolysaccharides; 11062-77-4/Superoxides; 7722-84-1/Hydrogen Peroxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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