| Deleterious effects of MRI on chondrocytes. | |
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MedLine Citation:
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PMID: 17804262 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To assess how magnetic fields (MFs), with or without concurrent radio frequency (RF), influence chondrocytes and knee joint repair, we applied an MF strength via magnetic resonance imaging (MRI) slightly greater than the frequently used dosage in the clinics and examined the effects of these treatments in vitro on human chondrocytes and in vivo in pigs. METHODS: Human chondrocytes were directly exposed to a 3-tesla (T) magnetic field (MF group) or a 3-T static magnetic field plus 125.3 MHz radio frequency (MF+RF group), and cell proliferation, apoptosis, cytosolic Ca2+ ([Ca2+]i) fluxes and expression of the apoptosis-related proteins of the treated cells were examined to assess the effects of the treatments. In the pig study, we examined the effects of the treatments on the recovery of surgically damaged pig knees. RESULTS: A 3-T static MF and RF suppressed cell growth and induced apoptosis through p53, p21, p27 and Bax protein expression. In the pig model, we found that MRI surveillance had a deleterious effect on the recovery of the damaged knee cartilage. CONCLUSION: Magnetic strength, with or without concurrent RF, suppressed chondrocyte growth in vitro and affected recovery of damaged knee cartilage in vivo in the pig model. These results may be specific to the parameters used in this study and may not apply to other situations, field strengths, forms of cartilage injury, or animal species. |
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Authors:
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C-H Hsieh; M-C Lee; J-J Tsai-Wu; M-H Chen; H-S Lee; H Chiang; C H Herbert Wu; C-C Jiang |
Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't Date: 2007-09-04 |
Journal Detail:
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Title: Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society Volume: 16 ISSN: 1063-4584 ISO Abbreviation: Osteoarthr. Cartil. Publication Date: 2008 Mar |
Date Detail:
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Created Date: 2008-03-07 Completed Date: 2008-07-24 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9305697 Medline TA: Osteoarthritis Cartilage Country: England |
Other Details:
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Languages: eng Pagination: 343-51 Citation Subset: IM |
Affiliation:
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Institute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan, ROC. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Animals Blotting, Western Calcium / metabolism* Cell Physiological Phenomena / radiation effects* Cells, Cultured Chondrocytes / cytology, metabolism, radiation effects* Collagen Type II / genetics DNA Fragmentation / radiation effects Disease Models, Animal Flow Cytometry Fura-2 Gene Expression / radiation effects Genes, p53 / genetics Humans Magnetic Resonance Imaging / adverse effects*, methods Middle Aged Regeneration / radiation effects* Stifle / injuries, physiology* Swine Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Collagen Type II; 7440-70-2/Calcium; 96314-98-6/Fura-2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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