Document Detail


Delayed secondary phase of peri-infarct depolarizations after focal cerebral ischemia: relation to infarct growth and neuroprotection.
MedLine Citation:
PMID:  14684862     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In focal cerebral ischemia, peri-infarct depolarizations (PIDs) cause an expansion of core-infarcted tissue into adjacent penumbral regions of reversible injury and have been shown to occur through 6 hr after injury. However, infarct maturation proceeds through 24 hr. Therefore, we studied PID occurrence through 72 hr after both transient and permanent middle cerebral artery occlusion (MCAo) via continuous DC recordings in nonanesthetized rats. PIDs occurred an average 13 times before reperfusion at 2 hr and then ceased for an average approximately 8 hr. After this quiescent period, PID activity re-emerged in a secondary phase, which reached peak incidence at 13 hr and consisted of a mean 52 PIDs over 2-24 hr. This phase corresponded to the period of infarct maturation; rates of infarct growth through 24 hr coincided with changes in PID frequency and peaked at 13 hr. In permanent MCAo, PIDs also occurred in a biphasic pattern with a mean of 78 events over 2-24 hr. Parameters of secondary phase PID incidence correlated with infarct volumes in transient and permanent ischemia models. The role of secondary phase PIDs in infarct development was further investigated in transient MCAo by treating rats with a high-affinity NMDA receptor antagonist at 8 hr after injury, which reduced post-treatment PID incidence by 57% and provided 37% neuroprotection. Topographic mapping with multielectrode recordings revealed multiple sources of PID initiation and patterns of propagation. These results suggest that PIDs contribute to the recruitment of penumbral tissue into the infarct core even after the restoration of blood flow and throughout the period of infarct maturation.
Authors:
Jed A Hartings; Michael L Rolli; X-C May Lu; Frank C Tortella
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of neuroscience : the official journal of the Society for Neuroscience     Volume:  23     ISSN:  1529-2401     ISO Abbreviation:  J. Neurosci.     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2003-12-19     Completed Date:  2004-01-16     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8102140     Medline TA:  J Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  11602-10     Citation Subset:  IM    
Affiliation:
Division of Neurosciences, Walter Reed Army Institute of Research, Silver Spring, Maryland 20910, USA. jed.hartings@amedd.army.mil
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain Ischemia / pathology*,  physiopathology*
Cell Death
Conotoxins / pharmacology
Cortical Spreading Depression
Electric Conductivity
Excitatory Amino Acid Antagonists / pharmacology
Infarction, Middle Cerebral Artery / pathology*,  physiopathology*
Kinetics
Male
Neuroprotective Agents / pharmacology
Rats
Rats, Sprague-Dawley
Receptors, N-Methyl-D-Aspartate / antagonists & inhibitors
Chemical
Reg. No./Substance:
0/Conotoxins; 0/Excitatory Amino Acid Antagonists; 0/Neuroprotective Agents; 0/Receptors, N-Methyl-D-Aspartate; 93438-65-4/conotoxin GV

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