Document Detail


Delayed response of QM- and DA/DAPI-fluorescence in C-heterochromatin of the small Japanese field mouse, Apodemus argenteus.
MedLine Citation:
PMID:  9200979     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The small Japanese field mouse Apodemus argenteus has the diploid chromosome number of 46, carrying rather large centromeric C-heterochromatin in most of the 44 autosomes and a large amount of C-heterochromatin in the sex chromosomes: the largest subtelocentric X was heterochromatic in almost two-fifth (whole short arm and proximal part of the long arm) of its entire length and the medium-sized acrocentric Y was totally heterochromatic. The C-heterochromatin (C-positive) areas, other than those of the Y and smallest three pairs, had a unique property of "delayed QM-fluorescence", which has not been reported to-date, showing dull QM-fluorescence immediately after exposure to blue light (BL), but gradually turning to bright fluorescence in a few minutes. The fluorescence intensity gradually decreased after attaining its peak, and finally became extinct. A similar pattern of fluorescence was also obtained in DA/DAPI-stained-X chromosome C-heterochromatin, but not in autosomal C-heterochromatin. No such dull-to-bright transition of QM-fluorescence could be obtained by CMA staining, for which the C-positive areas were apparently negative even after overexposure to BL. These facts indicate that the C-positive areas of A. argenteus showing dull-to-bright transition of QM-fluorescence contain A-T rich DNA. The delayed QM-fluorescence was found only in A. argenteus, in thirteen mammalian species so-far examined. Furthermore, this unique property of QM-fluorescence could be artificially altered to non-delayed ordinary type of fluorescence by sequentially pretreating the fixed chromosomes with hydrochloride and barium hydroxide solutions. The cytological implication of the delayed fluorescence in the C-heterochromatin of A. argenteus is briefly discussed.
Authors:
Y Obara; S Sasaki; Y Igarashi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Zoological science     Volume:  14     ISSN:  0289-0003     ISO Abbreviation:  Zool. Sci.     Publication Date:  1997 Feb 
Date Detail:
Created Date:  1997-08-07     Completed Date:  1997-08-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8702287     Medline TA:  Zoolog Sci     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  57-64     Citation Subset:  B    
Affiliation:
Department of Biology, Faculty of Science, Hirosaki University, Aomori, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromosome Banding
Fluorescence
Fluorescent Dyes*
Heterochromatin / chemistry*,  genetics
Indoles*
Mice
Muridae / genetics*
Quinacrine Mustard*
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/Heterochromatin; 0/Indoles; 4213-45-0/Quinacrine Mustard; 47165-04-8/DAPI

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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