| Delayed hypothermia preferentially increases expression of brain-derived neurotrophic factor exon III in rat hippocampus after asphyxial cardiac arrest. | |
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MedLine Citation:
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PMID: 15857665 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Brain-derived neurotrophic factor (BDNF) protein levels increase in rats treated with a regimen of delayed, mild hypothermia that improve neurological recovery after asphyxial cardiac arrest. BDNF transcription in rat brain involves at least five different BDNF exons (exons I-V) that produce four different varieties of mRNA, each containing exon V paired with one of exons I-IV. This study examined whether these different BDNF transcripts are differentially affected by cardiac arrest and by therapeutic hypothermia in rat hippocampus using a reverse transcription and PCR-based method. At 24 h after asphyxial cardiac arrest, transcripts containing exons I and III increased. In rats treated with hypothermia after cardiac arrest, transcripts containing exon III were further increased. No significant alterations in transcripts from exons II or IV were observed, though there was a trend for hypothermia to decrease message from these exons. These data suggest that hypothermia after cardiac arrest produces exon-specific changes in BDNF transcription. |
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Authors:
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Peter S Vosler; Eric S Logue; Melissa J Repine; Clifton W Callaway |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S. Date: 2005-01-07 |
Journal Detail:
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Title: Brain research. Molecular brain research Volume: 135 ISSN: 0169-328X ISO Abbreviation: Brain Res. Mol. Brain Res. Publication Date: 2005 Apr |
Date Detail:
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Created Date: 2005-04-28 Completed Date: 2005-07-25 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8908640 Medline TA: Brain Res Mol Brain Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 21-9 Citation Subset: IM |
Affiliation:
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Department of Emergency Medicine, University of Pittsburgh, 230 McKee Place, Suite 400, Pittsburgh, PA 15213, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Analysis of Variance Animals Asphyxia Blotting, Northern Brain-Derived Neurotrophic Factor / genetics, metabolism* Cyclophilins / genetics, metabolism Exons Fever / metabolism* Gene Expression Regulation / physiology* Heart Arrest / metabolism* Hippocampus / metabolism* Male RNA, Messenger / metabolism Rats Rats, Sprague-Dawley Reverse Transcriptase Polymerase Chain Reaction / methods Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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K02 NS02112/NS/NINDS NIH HHS; R01 NS46073/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Brain-Derived Neurotrophic Factor; 0/RNA, Messenger; EC 5.2.1.-/Cyclophilins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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