| Delayed expression of adeno-associated virus vector DNA. | |
| | |
MedLine Citation:
|
PMID: 10567839 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Two previous reports indicated that recombinant adeno-associated virus (rAAV) vectors were dependent on helper adenovirus (Ad) for efficient conversion of single-stranded (ss) rAAV DNA to the double-stranded (ds) form. This finding is somewhat paradoxical, however, since during a latent infection wild-type (wt)-AAV is rapidly converted to a ds form in the absence of Ad. Our hypothesis was that the effect observed in the previous studies was due to kinetic factors, i.e. to a relative delay in conversion to ds-DNA rather than to an absolute requirement for Ad. To test this, Hela cells were infected with a rAAV-CMV-green fluorescent protein (GFP) vector either in the presence or absence of Ad. Within the first 2 days, Ad infection resulted in a 4-fold increase in AAV vector expression and an augmentation of conversion to a ds-AAV DNA. By 6 days, however, the total number of GFP-expressing cells in the Ad-free culture had exceeded the original number in the Ad co-infected cells, and the conversion to ds-DNA episomes was substantial and ongoing. |
| | |
Authors:
|
S A Afione; J Wang; S Walsh; W B Guggino; T R Flotte |
Related Documents
:
|
6857999 - Proteins encoded near the adenovirus late messenger rna leader segments. 1870189 - Linker insertion mutations in the adenovirus preterminal protein that affect dna replic... 902319 - Further mapping of late adenovirus genes by cell-free translation of rna selected by hy... 513209 - In vitro association of empty adenovirus capsids with double-stranded dna. 2775459 - Association of adenovirus dna transcribed activity with nuclear matrix of host cells. 18180769 - Adeno-associated virus of a single-polarity dna genome is capable of transduction in vivo. 15542859 - Rna structural rearrangement via unwinding and annealing by the cyanobacterial rna heli... 2555539 - The essential 65-kilodalton dna-binding protein of herpes simplex virus stimulates the ... 12163469 - Nuclear dna helicase ii is recruited to ifn-alpha-activated transcription sites at pml ... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Intervirology Volume: 42 ISSN: 0300-5526 ISO Abbreviation: Intervirology Publication Date: 1999 |
Date Detail:
|
Created Date: 2000-01-06 Completed Date: 2000-01-06 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0364265 Medline TA: Intervirology Country: SWITZERLAND |
Other Details:
|
Languages: eng Pagination: 213-20 Citation Subset: IM |
Affiliation:
|
Department of Physiology and Pediatrics, Johns Hopkins University, Baltimore, Md., USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adenoviridae
/
physiology Cell Line, Transformed DNA, Recombinant / metabolism* Dependovirus / genetics, physiology* Genetic Vectors / genetics, physiology* Hela Cells Humans Time Factors Transfection Transformation, Genetic Tumor Cells, Cultured Virus Integration Virus Latency |
| Grant Support | |
ID/Acronym/Agency:
|
DK51809/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/DNA, Recombinant |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Social rank and sex-biased maternal investment in captive japanese macaques: behavioural and reprodu...
Next Document: PCR-RFLP-detected human papilloma virus infection in a group of senegalese women attending an STD cl...