Document Detail


Delayed chromosomal instability in lymphocytes of cancer patients after radiotherapy.
MedLine Citation:
PMID:  20353337     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: To assess possible delayed chromosomal instability (DCI) expressed as elevated chromatid breakage in cells containing previously formed chromosome type aberrations in cultured blood lymphocytes of cancer patients after radiotherapy (RT). MATERIALS AND METHODS: Twenty patients treated for uterine cancer with external Co(60) RT, without chemotherapy, were selected. Blood was taken before, 1-2 days after RT and one year later. Lymphocytes were cultured for 50 and 100 h. Metaphases were stained with fluorescence-plus-Giemsa and analysed for chromosome and chromatid aberrations in 1st (M1) and 3rd plus later (M3+) mitoses. RESULTS: RT caused a significant increase of radiation-specific chromosome aberrations in patients' lymphocytes together with DCI, which was observed as an excessive yield of cells containing both chromosome and chromatid aberrations (defined as C(acs&act)). This DCI passed successfully through mitoses in vitro, and at the end of RT a mean yield of 'extra' C(acs&act) was 3 x 10(-3) x cell(-1) amongst either M1 or M3+ cells. At the end of RT and one year later DCI in M1 lymphocytes appeared at random amongst patients, but some inter-individual variation was found for DCI presence in M3+ cells at both post-irradiation samplings. As time passed, the mean yield of lymphocytes exhibiting DCI decreased in vivo and one year after RT reached the pre-treatment level of 1 x 10(-3) x cell(-1). CONCLUSIONS: DCI was demonstrated in descendants of human lymphocytes after therapeutic irradiation. The effect diminished one year later, suggesting that the progeny of patients' irradiated stem cells did not produce new daughter lymphocytes exhibiting DCI during the studied post-irradiation period.
Authors:
Volodymyr A Vinnikov; Nataliya A Maznyk; David Lloyd
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of radiation biology     Volume:  86     ISSN:  1362-3095     ISO Abbreviation:  Int. J. Radiat. Biol.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-31     Completed Date:  2010-04-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8809243     Medline TA:  Int J Radiat Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  271-82     Citation Subset:  IM; S    
Affiliation:
Radiation Cytogenetics Laboratory, Institute for Medical Radiology AMS of Ukraine, Kharkiv, Ukraine. lrcg.imr@mail.ru
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MeSH Terms
Descriptor/Qualifier:
Aged
Chromosomal Instability / radiation effects*
Chromosome Aberrations / radiation effects*
Dose-Response Relationship, Radiation
Female
Humans
Lymphocytes / cytology,  metabolism,  radiation effects*
Middle Aged
Mitosis / radiation effects
Time Factors
Uterine Neoplasms / genetics*,  metabolism,  radionuclide imaging,  radiotherapy*

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