Document Detail


Delayed IgG2 humoral response in infants is not due to intrinsic T or B cell defects.
MedLine Citation:
PMID:  8921428     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The physiologically low or absent IgG2 responses of infants have been attributed to T or B cell functional immaturity. We have analyzed the capacity of adult and neonatal T lymphocytes to secrete IgG2 switch factor (IgG2-SF) and the capacity of neonatal B cells to respond to such factors. The IgG2-SF capacity was assessed on CD40-activated naive B cells, measuring IgG2 by ELISA in supernatants of cultures performed in the presence of IL-10. T cells secreted IgG2-SF together with IL-2 and IFN-gamma, after activation with a combination of anti-CD2, anti-CD28 and phorbol myristate acetate (Th1-like activation). In contrast, activation with anti-CD3 and anti-CD28, which yielded IL-4 and IL-10 but neither IL-2 nor IFN-gamma (Th2-like activation), did not result in the secretion of IgG2-SF. The supernatant of activated neonatal T cells contained IgG2-SF. Neonates' B cells produced almost as much IgG2 as did naive adult B cells. The effect of IgG2-SF was further demonstrated by its ability to induce 3-15% of CD40-activated naive B cells to express cytoplasmic IgG2 regardless of the presence of IL-10. This study demonstrates that: (i) IgG2 switch can be T cell dependent in humans, (ii) IgG2-SF is produced with Th1-like cytokines and (iii) low IgG2 responses in infants do not result from either an inability of T cells to produce IgG2-SF or an inability of B cells to undergo IgG2 switch in vitro.
Authors:
C Servet-Delprat; J M Bridon; O Djossou; S A Yahia; J Banchereau; F Brière
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International immunology     Volume:  8     ISSN:  0953-8178     ISO Abbreviation:  Int. Immunol.     Publication Date:  1996 Oct 
Date Detail:
Created Date:  1997-02-10     Completed Date:  1997-02-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8916182     Medline TA:  Int Immunol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1495-502     Citation Subset:  IM    
Affiliation:
Schering-Plough, Laboratory for Immunological Research, Dardilly, France.
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MeSH Terms
Descriptor/Qualifier:
Adult
B-Lymphocytes / immunology*,  metabolism*
Cytokines / biosynthesis
Fetal Blood / cytology
Humans
Immunoglobulin G / biosynthesis*
Infant, Newborn
Lymphocyte Activation / immunology
T-Lymphocytes / immunology*,  metabolism*
Time Factors
Chemical
Reg. No./Substance:
0/Cytokines; 0/Immunoglobulin G

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