| Delay of adenosine triphosphate depletion and hypoxanthine formation in rabbit lung after death. | |
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MedLine Citation:
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PMID: 8678649 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: If lungs could be retrieved for transplantation from non-heart-beating cadavers, the shortage of donors might be significantly alleviated. METHODS: Adenosine triphosphate (ATP) and hypoxanthine levels were measured postmortem in rabbit lungs comparing deflation (group 1), ventilation with room air (group 2), inflation with room air (group 3), ventilation with oxygen (group 4), ventilation with cooled air (group 5), deflation plus cadaver cooling (group 6), and cooling by pulmonary arterial flush (group 7). RESULTS: The level of ATP dropped to 25.9% and HYP increased elevenfold at 30 minutes in group 1 but remained constant during 24 hours in group 7. The ATP catabolism beyond 2 hours postmortem appeared less in group 2 compared with group 3 (3.58 +/- 1.24 versus 0.39 +/- 0.08 mumol/g dry weight for ATP and 3.03 +/- 0.49 versus 7.64 +/- 0.94 mumol/g dry weight for hypoxanthine at 24 hours, respectively; p < 0.05). Cadaver cooling significantly slowed ATP catabolism. Changes in ATP level were similar in groups 2, 4, and 5. CONCLUSIONS: These data suggest that in the non-heart-beating cadaver (1) cooling, ventilation, and inflation can delay ATP catabolism; (2) postmortem ventilation but not inflation for more than 2 hours will inhibit further ATP breakdown; (3) ventilation with either oxygen or cooled air is not more beneficial than room air ventilation; and (4) cold flush more than cadaver cooling will prevent ATP depletion. |
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Authors:
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D E Van Raemdonck; N C Jannis; F R Rega; P R de Leyn; W J Flameng; T E Lerut |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Annals of thoracic surgery Volume: 62 ISSN: 0003-4975 ISO Abbreviation: Ann. Thorac. Surg. Publication Date: 1996 Jul |
Date Detail:
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Created Date: 1996-08-14 Completed Date: 1996-08-14 Revised Date: 2010-03-24 |
Medline Journal Info:
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Nlm Unique ID: 15030100R Medline TA: Ann Thorac Surg Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 233-40; discussion 240-1 Citation Subset: AIM; IM |
Affiliation:
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Center for Experimental Surgery and Anesthesiology, Katholieke Universiteit Leuven, Belgium. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Diphosphate
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metabolism Adenosine Monophosphate / metabolism Adenosine Triphosphate / metabolism* Animals Biopsy Cadaver Chromatography, High Pressure Liquid Hypoxanthine Hypoxanthines / metabolism* Lung / metabolism*, pathology Lung Transplantation Organ Preservation Postmortem Changes* Rabbits Time Factors Tissue Donors |
| Chemical | |
Reg. No./Substance:
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0/Hypoxanthines; 56-65-5/Adenosine Triphosphate; 58-64-0/Adenosine Diphosphate; 61-19-8/Adenosine Monophosphate; 68-94-0/Hypoxanthine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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