Document Detail


Dehydroglyasperin C, a component of liquorice, attenuates proliferation and migration induced by platelet-derived growth factor in human arterial smooth muscle cells.
MedLine Citation:
PMID:  23298457     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Liquorice is one of the botanicals used frequently as a traditional medicine in the West and in the East. Platelet-derived growth factor (PDGF)-BB is involved in the development of CVD by inducing abnormal proliferation and migration of vascular smooth muscle cells. In our preliminary study, dehydroglyasperin C (DGC), an active compound of liquorice, showed strong antioxidant activity. Since phytochemicals with antioxidant activities showed beneficial effects on chronic inflammatory diseases, the present study aimed to investigate the effects of DGC on PDGF-induced proliferation and migration of human aortic smooth muscle cells (HASMC). Treatment of HASMC with DGC for 24 h significantly decreased PDGF-induced cell number and DNA synthesis in a dose-dependent manner without any cytotoxicity, as demonstrated by the 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide test and thymidine incorporation. Upon cell cycle analysis, DGC blocked the PDGF-induced progression through the G0/G1 to S phase of the cell cycle, and down-regulated the expression of cyclin-dependent kinase (CDK); 2, cyclin E, CDK4 and cyclin D1. Furthermore, DGC significantly attenuated PDGF-stimulated phosphorylation of PDGF receptor-β, phospholipase C-γ1, AKT and extracellular-regulated kinase 1/2, and DGC inhibited cell migration and the dissociation of actin filaments by PDGF. In a rat vascular balloon injury model, DGC suppressed an excessive reduction in luminal diameters and neointimal formation compared with the control group. These results demonstrate the mechanistic basis for the prevention of CVD and the potential therapeutic properties of DGC.
Authors:
Hyo Jung Kim; Byung-Yoon Cha; In Sil Park; Ji Sun Lim; Je-Tae Woo; Jong-Sang Kim
Related Documents :
23401567 - Small molecule inhibition of p38 map kinase extends the replicative life span of human ...
21543897 - Ron (mst1r) is a novel prognostic marker and therapeutic target for gastroesophageal ad...
24016057 - Vermelhotin, an anti-inflammatory agent, suppresses nitric oxide production in raw 264....
6933467 - Phenotypic evolution of cells resistant to bromodeoxyuridine.
7542197 - Phosphotyrosine-dependent association between cd22 and protein tyrosine phosphatase 1c.
16822837 - The ste20/germinal center kinase pod6 interacts with the ndr kinase cot1 and is involve...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-9
Journal Detail:
Title:  The British journal of nutrition     Volume:  -     ISSN:  1475-2662     ISO Abbreviation:  Br. J. Nutr.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372547     Medline TA:  Br J Nutr     Country:  -    
Other Details:
Languages:  ENG     Pagination:  1-10     Citation Subset:  -    
Affiliation:
School of Applied Biosciences, Food Science and Biotechnology, BK21 Research Team for Developing Functional Health Food Materials, Kyungpook National University, Daegu 702-701, Republic of Korea.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Geniposidic acid protects against D-galactosamine and lipopolysaccharide-induced hepatic failure in ...
Next Document:  Obese youths are not more likely to become depressed, but depressed youths are more likely to become...