| Dehydrogenation of ribitol with Gluconobacter oxydans: production and stability of L-ribulose. | |
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MedLine Citation:
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PMID: 16650498 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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l-Ribulose is an important chiral lead molecule used for the synthesis of, among others, l-ribose, a high-value rare sugar used in the preparation of antiviral drugs. These drugs--nucleoside-analogues--gain importance in the treatment of severe viral diseases, like those caused by the HIV or hepatitis virus. In this study, factors that may have an impact on l-ribulose production with Gluconobacter oxydans and on the stability of l-ribulose were investigated. A bioconversion-type process, using washed resting cells, was chosen to produce l-ribulose from ribitol. In this process, the cell production and bioconversion phase were separated. The former was first optimized and a maximum cell mass of 1.5 g CDWL(-1) could be produced. For the bioconversion phase, the aeration level of the system proved to be one of the most critical factors; a maximal production rate of 15.7 g L(-1)h(-1) or 5.9 g(g CDW)(-1)h(-1) of l-ribulose could be reached. Furthermore, resting cells were found capable of completely converting ribitol solutions of up to 300 g L(-1) within 30 h, although the kinetics indicated a rather low affinity of the dehydrogenase enzymes for the substrate. |
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Authors:
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Cassandra De Muynck; Catarina Pereira; Wim Soetaert; Erick Vandamme |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2006-05-02 |
Journal Detail:
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Title: Journal of biotechnology Volume: 125 ISSN: 0168-1656 ISO Abbreviation: J. Biotechnol. Publication Date: 2006 Sep |
Date Detail:
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Created Date: 2006-08-21 Completed Date: 2006-12-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8411927 Medline TA: J Biotechnol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 408-15 Citation Subset: IM |
Affiliation:
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Laboratory of Industrial Microbiology and Biocatalysis, Department of Biochemical and Microbial Technology, Ghent University, Coupure links 653, B-9000 Gent, Belgium. Cassandra.DeMuynck@UGent.be <Cassandra.DeMuynck@UGent.be> |
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetobacter
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enzymology,
growth & development Biomass Carbon / supply & distribution Cell Count Drug Stability Gluconobacter oxydans / enzymology*, growth & development Hydrogen-Ion Concentration Models, Biological Oxidoreductases / metabolism*, pharmacokinetics Oxygen / pharmacology Pentoses / biosynthesis*, metabolism, pharmacokinetics Ribitol / metabolism*, pharmacokinetics Sugar Alcohol Dehydrogenases / metabolism, pharmacokinetics Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Pentoses; 488-81-3/Ribitol; 5556-48-9/ribulose; 7440-44-0/Carbon; 7782-44-7/Oxygen; EC 1.-/Oxidoreductases; EC 1.1.-/Sugar Alcohol Dehydrogenases; EC 1.1.1.-/L-ribose reductase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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