| Dehydroepiandrosterone modulates survival and immune functions during sepsis depending on the way of drug administration. | |
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MedLine Citation:
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PMID: 20843286 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Administration of dehydroepiandrosterone (DHEA) has been demonstrated to improve survival and cellular immune functions during systemic inflammation. Although there is evidence that the route of drug application may profoundly affect the DHEA-induced effects the impact of this parameter remains to be established. MATERIALS AND METHODS: Male NMRI mice were subjected to sham-operation (laparotomy) or sepsis (cecal ligation and puncture). Animals received saline or DHEA (20 mg/kg/day) given either subcutaneously, intravenously, or intraperitoneally. Termination of animals was performed 48 hrs after induction of sepsis in order to monitor splenocyte proliferation ((3)H-thymidine incorporation assay), splenocyte apoptosis (Annexin V binding capacity), and cytokine release (IL-1β and IL-6, ELISA). RESULTS: Subcutaneous DHEA administration improved the survival rate of septic mice 48 hrs after induction of CLP (75% vs. 47%). This effect was paralleled by a restoration of splenocyte proliferation, a decreased cellular apoptosis rate of splenocytes, and an attenuation of pro-inflammatory cytokine release. In contrast, no significant effects on the survival rate or cellular immune functions were observed following intravenous or intraperitoneal DHEA administration. CONCLUSIONS: Subcutaneous administration of DHEA induced an increased survival rate and improved cellular immune functions in septic mice. In contrast, no comparable effects were noticed following intravenous or intraperitoneal administration of DHEA. |
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Authors:
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Daniel Schmitz; Philipp Kobbe; Alexander Wegner; Florian Hammes; Reiner Oberbeck |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Medicinal chemistry (Shāriqah (United Arab Emirates)) Volume: 6 ISSN: 1875-6638 ISO Abbreviation: Med Chem Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-10-14 Completed Date: 2011-02-16 Revised Date: 2012-03-14 |
Medline Journal Info:
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Nlm Unique ID: 101240303 Medline TA: Med Chem Country: Netherlands |
Other Details:
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Languages: eng Pagination: 229-32 Citation Subset: IM |
Affiliation:
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Dept. Trauma Surgery, University Hospital of Essen, Essen, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects Cell Proliferation / drug effects Cytokines / immunology, secretion Dehydroepiandrosterone / administration & dosage, pharmacology* Disease Models, Animal Dose-Response Relationship, Drug Inflammation / drug therapy*, immunology Injections, Intraperitoneal Injections, Intravenous Injections, Subcutaneous Male Mice Sepsis / drug therapy*, immunology Survival Rate |
| Chemical | |
Reg. No./Substance:
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0/Cytokines; 53-43-0/Dehydroepiandrosterone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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