Document Detail

Dehydration modifies guanidino compound concentrations in the different zones of the rat kidney.
MedLine Citation:
PMID:  11976926     Owner:  NLM     Status:  MEDLINE    
Guanidino compounds (GCs) related to arginine (Arg) are unevenly distributed along the cortico-papillary axis of the rat kidney. Inasmuch as the concentration of alpha-keto-delta-guanidinovaleric acid (alpha-keto-delta-GVA), guanidinosuccinic acid (GSA), creatinine (CTN), gamma-guanidinobutyric acid (gamma-GBA) and methylguanidine (MG) increased steeply along the inner medulla in parallel to the urea and osmotic gradients, the question arose as to whether dehydration enhances their renal content and distribution. To examine this possibility, adult male rats were dehydrated by removing the drinking water for 24 or 48 h. The kidneys were sliced and cut in seven sections along the cortico-papillary axis. Twelve GCs were determined by liquid chromatography in each renal zone. Dehydration modified GC concentrations and regional distribution. The renal content of Arg, guanidine and MG was decreased while that of alpha-keto-delta-GVA, gamma-GBA, alpha- N-acetyl-arginine and homoarginine remained unchanged. In contrast, GSA, guanidinoacetic acid (GAA), creatine (CT), CTN and beta-guanidinopropionic acid (beta-GPA) concentrations were enhanced significantly in different renal zones after 24 and 48 h dehydration. In addition, the tissue level of GCs supplying energy, such as CT and beta-GPA, the precursor of CT (GAA) and its metabolite (CTN) were enhanced under dehydration. Arg and CT account for 80-90% of the GCs located in the renal cortex. Variations of some GC levels under dehydration may modify enzyme activities, renal metabolism and cell function.
Olivier Levillain; Bart Marescau; Ilse Possemiers; Paul De Deyn
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2002-03-14
Journal Detail:
Title:  Pflügers Archiv : European journal of physiology     Volume:  444     ISSN:  0031-6768     ISO Abbreviation:  Pflugers Arch.     Publication Date:  2002 May 
Date Detail:
Created Date:  2002-04-26     Completed Date:  2002-11-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0154720     Medline TA:  Pflugers Arch     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  143-52     Citation Subset:  IM    
Faculté de Médecine Lyon R.T.H. Laennec, Laboratoire de Physiologie Métabolique et Rénale, INSERM U 499, 12 Rue G. Paradin, 69372 LYON Cedex 08, France.
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MeSH Terms
Arginine / metabolism
Chromatography, Ion Exchange
Creatine / metabolism
Creatinine / metabolism
Dehydration / metabolism*
Energy Metabolism / physiology
Guanidines / metabolism*
Kidney / metabolism*
Kidney Cortex / metabolism
Kidney Medulla / metabolism
Osmolar Concentration
Rats, Sprague-Dawley
Spectrometry, Fluorescence
Urea / metabolism
Reg. No./Substance:
0/Guanidines; 57-00-1/Creatine; 57-13-6/Urea; 60-27-5/Creatinine; 74-79-3/Arginine

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