Document Detail


Degree of protein deficiency affects the extent of the depression of the antioxidative enzyme activities and the enhancement of tissue lipid peroxidation in rats.
MedLine Citation:
PMID:  8487090     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To investigate the effects of various dietary protein levels on tissue lipid peroxidation and antioxidative enzyme activities, four groups of Long-Evans male weanling rats were fed a 6, 8, 12 or 20% lactalbumin diet for 6 wk. The red blood cell in vitro spontaneous hemolysis values were 28.6, 24.9, 18.9 and 14.1% for the rats fed the 6, 8, 12 and 20% lactalbumin diets, respectively. The thiobarbituric acid-reactive substances (TBARS) in plasma, RBC, liver, kidney, muscle, lung, spleen and heart were lowest in the group fed the 20% lactalbumin diet. Rats fed the diet containing 12% lactalbumin had significantly greater TBARS in plasma, spleen, kidney and muscle compared with rats fed the 20% lactalbumin diet. Rats fed the 8% lactalbumin diet had markedly higher TBARS levels (P < 0.05) in all tissues examined than did those fed the 12% lactalbumin diet. The TBARS in all eight tissues of the rats fed the 6% lactalbumin diet were even higher than those of rats fed the 8% lactalbumin diet (P < 0.05). The activities of glutathione peroxidase, superoxide dismutase and glucose-6-phosphate dehydrogenase in RBC were gradually lowered (P < 0.05) when the dietary protein level was decreased stepwise. The activities of these antioxidative enzymes in liver also were reduced significantly when dietary protein level decreased from 20 to 12% and then to 8%. These enzyme activities in the rats fed the diet containing 6% lactalbumin generally were not different from those of the rats fed 8% lactalbumin.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
C J Huang; M L Fwu
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of nutrition     Volume:  123     ISSN:  0022-3166     ISO Abbreviation:  J. Nutr.     Publication Date:  1993 May 
Date Detail:
Created Date:  1993-06-07     Completed Date:  1993-06-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0404243     Medline TA:  J Nutr     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  803-10     Citation Subset:  IM    
Affiliation:
Department of Agricultural Chemistry, National Taiwan University, Taipei, Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Dietary Proteins / metabolism
Erythrocytes / enzymology,  metabolism*
Glucosephosphate Dehydrogenase / analysis*
Glutathione Peroxidase / metabolism
Lipid Peroxidation / physiology*
Liver / enzymology,  metabolism*
Male
Nutritional Status
Oxidation-Reduction
Protein Deficiency / enzymology,  metabolism*
Rats
Rats, Inbred Strains
Superoxide Dismutase / metabolism
Thiobarbituric Acid Reactive Substances / metabolism
Chemical
Reg. No./Substance:
0/Dietary Proteins; 0/Thiobarbituric Acid Reactive Substances; EC 1.1.1.49/Glucosephosphate Dehydrogenase; EC 1.11.1.9/Glutathione Peroxidase; EC 1.15.1.1/Superoxide Dismutase

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