Document Detail


Degradation of very long chain dicarboxylic polyunsaturated fatty acids in mouse hepatocytes, a peroxisomal process.
MedLine Citation:
PMID:  18619556     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Polyunsaturated fatty acids can be omega-oxidized to dicarboxylic polyunsaturated fatty acids (DC-PUFA), bioactive compounds which cause vasodilatation and activation of PPARalpha and gamma. DC-PUFA can be shortened by beta-oxidation, and to determine whether mitochondria and/or peroxisomes are responsible for this degradation 20-carboxy-[1-(14)C]-eicosatetraenoic acid (20-COOH-AA) was synthesized and given to hepatocytes from mouse models with peroxisomal dysfunctions. In contrast to wild type cells, hepatocytes from mice with liver-selective elimination of peroxisomes, due to Pex5p deficiency, failed to produce (14)CO(2) and labeled acid-soluble oxidation products, indicating that peroxisomes are involved in the degradation of 20-COOH-AA. Subsequently, the oxidation of 20-COOH-AA was analyzed in hepatocytes lacking multifunctional protein 1 (MFP1) or MFP2, key enzymes of the peroxisomal beta-oxidation. Degradation of 20-COOH-AA was partially impaired in MFP1, but not in MFP2 knockout hepatocytes. Taken together, peroxisomes and not mitochondria are the site of beta-oxidation of DC-PUFA, and MFP1 is involved in this process.
Authors:
Su Duy Nguyen; Myriam Baes; Paul P Van Veldhoven
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-06-19
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1781     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-08-11     Completed Date:  2008-10-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  400-5     Citation Subset:  IM    
Affiliation:
Laboratory of Lipid Biochemistry and Protein Interactions, Department of Molecular Cell Biology, KU Leuven, Leuven, Belgium.
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MeSH Terms
Descriptor/Qualifier:
Animals
Fatty Acids, Unsaturated / analysis,  biosynthesis,  metabolism*
Hepatocytes / metabolism*
Mice
Mice, Knockout
Oxidation-Reduction
Peroxisomes / metabolism*
Receptors, Cytoplasmic and Nuclear / metabolism
Time Factors
Chemical
Reg. No./Substance:
0/Fatty Acids, Unsaturated; 0/Receptors, Cytoplasmic and Nuclear; 0/peroxisome-targeting signal 1 receptor

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