| Degradation of soluble and insoluble proteins by Bacteroides amylophilus protease and by rumen microorganisms. | |
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MedLine Citation:
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PMID: 6989794 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Various soluble and insoluble proteins (6.25 mg) were incubated at 37 C with partially purified protease from Bacteroides amylophilus (156 micrograms) in 2.0 ml of .1 M potassium phosphate buffer, pH 7.6, for 2, 4, 6 and 18 hr, and the liberated amino acids were determined by the ninhydrin method. Results showed that (1) although soluble, serum albumin and ribonuclease A were resistant to hydrolysis; (2) soluble and insoluble proteins of soybean meal were hydrolyzed at almost identical rates; (3) soluble proteins from soybean meal, rapeseed meal and casein were hydrolyzed at different rates, and (4) treatment of resistant proteins (serum albumin, ribonuclease A and insoluble fish meal and rapeseed meal proteins) with mercaptoethanol in 8 M urea or oxidation with performic acid rendered these proteins susceptible to hydrolysis. It is concluded that (1) solubility or insolubility of a protein is not by itself an indication of the protein's resistance or susceptibility to hydrolysis by rumen bacterial protease; (2) structural characteristics of the properties which renders feed protein resistant to degradation is the presence of crosslinking disulfide bonds. |
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Authors:
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S Mahadevan; J D Erfle; F D Sauer |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of animal science Volume: 50 ISSN: 0021-8812 ISO Abbreviation: J. Anim. Sci. Publication Date: 1980 Apr |
Date Detail:
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Created Date: 1980-07-12 Completed Date: 1980-07-12 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 8003002 Medline TA: J Anim Sci Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 723-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bacteroides / metabolism* Caseins / metabolism Cattle / metabolism* Peptide Hydrolases / metabolism* Proteins / metabolism* Rumen / metabolism*, microbiology Solubility Soybeans |
| Chemical | |
Reg. No./Substance:
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0/Caseins; 0/Proteins; EC 3.4.-/Peptide Hydrolases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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