Document Detail

Degradation of soluble and insoluble proteins by Bacteroides amylophilus protease and by rumen microorganisms.
MedLine Citation:
PMID:  6989794     Owner:  NLM     Status:  MEDLINE    
Various soluble and insoluble proteins (6.25 mg) were incubated at 37 C with partially purified protease from Bacteroides amylophilus (156 micrograms) in 2.0 ml of .1 M potassium phosphate buffer, pH 7.6, for 2, 4, 6 and 18 hr, and the liberated amino acids were determined by the ninhydrin method. Results showed that (1) although soluble, serum albumin and ribonuclease A were resistant to hydrolysis; (2) soluble and insoluble proteins of soybean meal were hydrolyzed at almost identical rates; (3) soluble proteins from soybean meal, rapeseed meal and casein were hydrolyzed at different rates, and (4) treatment of resistant proteins (serum albumin, ribonuclease A and insoluble fish meal and rapeseed meal proteins) with mercaptoethanol in 8 M urea or oxidation with performic acid rendered these proteins susceptible to hydrolysis. It is concluded that (1) solubility or insolubility of a protein is not by itself an indication of the protein's resistance or susceptibility to hydrolysis by rumen bacterial protease; (2) structural characteristics of the properties which renders feed protein resistant to degradation is the presence of crosslinking disulfide bonds.
S Mahadevan; J D Erfle; F D Sauer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of animal science     Volume:  50     ISSN:  0021-8812     ISO Abbreviation:  J. Anim. Sci.     Publication Date:  1980 Apr 
Date Detail:
Created Date:  1980-07-12     Completed Date:  1980-07-12     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8003002     Medline TA:  J Anim Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  723-8     Citation Subset:  IM    
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MeSH Terms
Bacteroides / metabolism*
Caseins / metabolism
Cattle / metabolism*
Peptide Hydrolases / metabolism*
Proteins / metabolism*
Rumen / metabolism*,  microbiology
Reg. No./Substance:
0/Caseins; 0/Proteins; EC 3.4.-/Peptide Hydrolases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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