Document Detail

Degradation of oxalate in rats implanted with immobilized oxalate oxidase.
MedLine Citation:
PMID:  3943604     Owner:  NLM     Status:  MEDLINE    
Accumulation of oxalate leads to hyperoxaluria and calcium oxalate nephrolithiasis in man. Since oxalate is a metabolic end product in mammals, the feasibility of its enzymic degradation has been tested in vivo in rats by administering exogenous oxalate oxidase. Oxalate oxidase, isolated from banana fruit peels, in its native form was found to be non-active at the physiological pH of the recipient animal. However, its functional viability in the recipient animal was ensured by its prior binding with ethylenemaleic anhydride, thus shifting its pH activity curve towards the alkaline range. Rats implanted with dialysis membrane capsules containing such immobilized oxalate oxidase in their peritoneal cavities effectively metabolized intraperitoneally injected [14C]oxalate as well as its precursor [14C]glyoxalate. The implantation of capsules containing coentrapped multienzyme preparations of oxalate oxidase, catalase and peroxidase led to a further degradation of administered [14C]oxalate in rats.
K G Raghavan; U Tarachand
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  FEBS letters     Volume:  195     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1986 Jan 
Date Detail:
Created Date:  1986-03-06     Completed Date:  1986-03-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  101-5     Citation Subset:  IM    
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MeSH Terms
Dialysis / instrumentation
Enzymes, Immobilized / metabolism
Hydrogen-Ion Concentration
Oxalates / metabolism*,  urine
Oxidoreductases / administration & dosage,  metabolism*
Peritoneal Cavity / enzymology
Reg. No./Substance:
0/Enzymes, Immobilized; 0/Oxalates; EC 1.-/Oxidoreductases; EC oxidase

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