Document Detail

Degradation and intrahepatic compatibility of albumin-heparin conjugate microspheres.
MedLine Citation:
PMID:  7948576     Owner:  NLM     Status:  MEDLINE    
The in vitro degradation properties of glutaraldehyde cross-linked albumin and albumin-heparin conjugate microspheres (AMS and AHCMS respectively) were evaluated using light microscopy, turbidity measurements and heparin release determinations, showing that the microspheres are degraded by proteolytic enzymes such as trypsin, proteinase K and lysosomal enzymes. The degradation rate was inversely related to the cross-link density of the microspheres. After intrahepatic administration of AHCMS, cross-linked with 0.5% glutaraldehyde, to male Wag/Rij rats by injection into a mesenteric vein (intravenoportal: i.v.p.), the microspheres were entrapped in the hepatic vascular system. The AHCMS were entrapped within terminal portal veins predominantly at the periphery of the liver. The AHCMS were degraded by cellular enzymatic processes within 2 wk after injection, with a half life of approximately 1 d. Biocompatibility of AHCMS and adriamycin-loaded AHCMS was evaluated by histological assessment of the mitotic activity of liver parenchyma and inflammatory response, and by determination of liver damage marker enzymes during 4 wk after administration. Liver damage marker enzymes were not increased compared with controls, nor were adverse effects observed upon histological examination. There was no difference in response between empty and adriamycin-loaded AHCMS.
H F Cremers; R F Wolf; E H Blaauw; J M Schakenraad; K H Lam; P Nieuwenhuis; R Verrijk; G Kwon; Y H Bae; S W Kim
Related Documents :
21902626 - Substrate reduction therapies for mucopolysaccharidoses.
19447296 - Concerted action of the cytosolic sulfotransferase, sult1a3, and catechol-o-methyltrans...
17662596 - Interactions of the human cytosolic sulfotransferases and steroid sulfatase in the meta...
14733596 - Dendritic polyglycerol sulfates as new heparin analogues and potent inhibitors of the c...
6447156 - Increased permeability of the glomerular basement membrane to ferritin after removal of...
22173936 - Characterisation of anthocyanidin reductase from shuchazao green tea.
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomaterials     Volume:  15     ISSN:  0142-9612     ISO Abbreviation:  Biomaterials     Publication Date:  1994 Jun 
Date Detail:
Created Date:  1994-12-06     Completed Date:  1994-12-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  577-85     Citation Subset:  IM    
Department of Chemical Technology, University of Twente, Enschede, The Netherlands.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Albumins / chemical synthesis,  metabolism*
Biocompatible Materials / metabolism*
Cross-Linking Reagents
Doxorubicin / administration & dosage
Heparin / chemical synthesis,  metabolism*
Injections, Intravenous
Liver / enzymology,  metabolism*,  pathology
Reg. No./Substance:
0/Albumins; 0/Biocompatible Materials; 0/Cross-Linking Reagents; 111-30-8/Glutaral; 23214-92-8/Doxorubicin; 9005-49-6/Heparin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Influence of the structure of support microspheres on leucocyte activation by RGDS-carrying microsph...
Next Document:  Stability and storage conditions of NADH-cytochrome b5 reductase cross-linked into gelatin by chromi...