Document Detail


Degradation and intrahepatic compatibility of albumin-heparin conjugate microspheres.
MedLine Citation:
PMID:  7948576     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The in vitro degradation properties of glutaraldehyde cross-linked albumin and albumin-heparin conjugate microspheres (AMS and AHCMS respectively) were evaluated using light microscopy, turbidity measurements and heparin release determinations, showing that the microspheres are degraded by proteolytic enzymes such as trypsin, proteinase K and lysosomal enzymes. The degradation rate was inversely related to the cross-link density of the microspheres. After intrahepatic administration of AHCMS, cross-linked with 0.5% glutaraldehyde, to male Wag/Rij rats by injection into a mesenteric vein (intravenoportal: i.v.p.), the microspheres were entrapped in the hepatic vascular system. The AHCMS were entrapped within terminal portal veins predominantly at the periphery of the liver. The AHCMS were degraded by cellular enzymatic processes within 2 wk after injection, with a half life of approximately 1 d. Biocompatibility of AHCMS and adriamycin-loaded AHCMS was evaluated by histological assessment of the mitotic activity of liver parenchyma and inflammatory response, and by determination of liver damage marker enzymes during 4 wk after administration. Liver damage marker enzymes were not increased compared with controls, nor were adverse effects observed upon histological examination. There was no difference in response between empty and adriamycin-loaded AHCMS.
Authors:
H F Cremers; R F Wolf; E H Blaauw; J M Schakenraad; K H Lam; P Nieuwenhuis; R Verrijk; G Kwon; Y H Bae; S W Kim
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomaterials     Volume:  15     ISSN:  0142-9612     ISO Abbreviation:  Biomaterials     Publication Date:  1994 Jun 
Date Detail:
Created Date:  1994-12-06     Completed Date:  1994-12-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  577-85     Citation Subset:  IM    
Affiliation:
Department of Chemical Technology, University of Twente, Enschede, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Albumins / chemical synthesis,  metabolism*
Animals
Biocompatible Materials / metabolism*
Cross-Linking Reagents
Doxorubicin / administration & dosage
Glutaral
Heparin / chemical synthesis,  metabolism*
Injections, Intravenous
Liver / enzymology,  metabolism*,  pathology
Male
Microspheres*
Rats
Chemical
Reg. No./Substance:
0/Albumins; 0/Biocompatible Materials; 0/Cross-Linking Reagents; 111-30-8/Glutaral; 23214-92-8/Doxorubicin; 9005-49-6/Heparin

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