Document Detail


Degradation of hyaluronate by Streptococcus intermedius strain UNS 35.
MedLine Citation:
PMID:  7966219     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Streptococcus intermedius strain UNS 35, a brain abscess isolate, produced extracellular hyaluronidase when grown in brain heart infusion broth. Chemical assays with this enzyme indicated that hyaluronate depolymerisation resulted in the formation of carbohydrate moieties with N-acetylglucosamine at the reducing terminal and containing an unsaturated carbon-carbon double bond. The nature of the products of this hyaluronidase were investigated further by high-field (400 MHz) proton (1H) NMR spectroscopy. Treatment of hyaluronate with the enzyme resulted in a series of new, sharp resonances in spectra (acetamido methyl group singlets located at 2.03 and 2.07 ppm, sugar ring proton multiplets in the 3.5-4.2 ppm chemical shift range, and doublets at 5.16 and 5.87 ppm) characteristic of low-M(r) oligosaccharide species, predominantly those containing glucuronosyl residues with delta 4,5-carbon-carbon double bonds. Comparison of spectra acquired from hyaluronidase-treated samples with that of an authentic sample of 4-deoxy-L-threo-hex-4-enopyranosyluronic-acid-N-acetylglucosamine (delta UA GlcNAc) indicated that this disaccharide was a major product arising from the actions of this enzyme. When used in minimal media, hyaluronate supported growth of S. intermedius, with lactate as the major metabolic end-product.
Authors:
K A Homer; M C Grootveld; J Hawkes; D P Naughton; D Beighton
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of medical microbiology     Volume:  41     ISSN:  0022-2615     ISO Abbreviation:  J. Med. Microbiol.     Publication Date:  1994 Dec 
Date Detail:
Created Date:  1994-12-21     Completed Date:  1994-12-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0224131     Medline TA:  J Med Microbiol     Country:  SCOTLAND    
Other Details:
Languages:  eng     Pagination:  414-22     Citation Subset:  IM    
Affiliation:
Royal College of Surgeons, Department of Dental Sciences, King's College School of Medicine and Dentistry, Faculty of Clinical Dentistry, London.
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MeSH Terms
Descriptor/Qualifier:
Carbohydrate Sequence
Humans
Hyaluronic Acid / chemistry,  metabolism*
Hyaluronoglucosaminidase / metabolism*
Hydrogen-Ion Concentration
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Oligosaccharides / chemistry
Streptococcus / enzymology*,  growth & development
Chemical
Reg. No./Substance:
0/Oligosaccharides; 9004-61-9/Hyaluronic Acid; EC 3.2.1.35/Hyaluronoglucosaminidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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