Document Detail


Degradation of human collagen isoforms by Clostridium collagenase and the effects of degradation products on cell migration.
MedLine Citation:
PMID:  20529148     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Clostridium collagenase has been widely used in biomedical research to dissociate tissues and isolate cells; and, since 1965, as a therapeutic drug for the removal of necrotic wound tissues. Previous studies found that purified collagenase-treated extracellular matrix stimulated cellular response to injury and increased cell proliferation and migration. This article presents an in vitro study investigating the digestive ability of Clostridium collagenase on human collagen types I, III, IV, V and VI. Our results showed that Clostridium collagenase displays proteolytic power to digest all these types of human collagen, except type VI. The degradation products derived were tested in cell migration assays using human keratinocytes (gold surface migration assay) and fibroblasts (chemotaxis cell migration assay). Clostridium collagenase itself and the degradation products of type I and III collagens showed an increase in keratinocyte and fibroblast migration, type IV-induced fibroblast migration only, and the remainder showed no effects compared with the control. The data indicate that Clostridium collagenase can effectively digest collagen isoforms that are present in necrotic wound tissues and suggest that collagenase treatment provides several mechanisms to enhance cell migration: collagenase itself and collagen degradation products.
Authors:
Lei Shi; Ryan Ermis; Anastacia Garcia; Dale Telgenhoff; Duncan Aust
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  International wound journal     Volume:  7     ISSN:  1742-481X     ISO Abbreviation:  Int Wound J     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-06-10     Completed Date:  2010-09-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101230907     Medline TA:  Int Wound J     Country:  England    
Other Details:
Languages:  eng     Pagination:  87-95     Citation Subset:  IM    
Affiliation:
Research & Development, Healthpoint Ltd, Fort Worth, TX 76107, USA. lei.shi@healthpoint.com
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MeSH Terms
Descriptor/Qualifier:
Cell Migration Assays
Cell Movement / drug effects*
Cell Proliferation / drug effects*
Clostridium / enzymology
Fibrillar Collagens / drug effects*
Fibroblasts / drug effects*
Humans
Keratinocytes / drug effects*
Microbial Collagenase / pharmacology*
Chemical
Reg. No./Substance:
0/Fibrillar Collagens; EC 3.4.24.3/Microbial Collagenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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