Document Detail


Degradation of forage chicory by ruminal fibrolytic bacteria.
MedLine Citation:
PMID:  18713292     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS: Determine the susceptibility of forage chicory (Cichorium intybus L.) to degradation by ruminal fibrolytic bacteria and measure the effects on cell-wall pectic polysaccharides. METHODS AND RESULTS: Large segments of fresh forage chicory were degraded in vitro by Lachnospira multiparus and Fibrobacter succinogenes, but not by Ruminococcus flavefaciens or Butyrivibrio hungatei. Cell-wall pectins were degraded extensively (95%) and rapidly by L. multiparus with a simultaneous release of uronic acids and the pectin-derived neutral monosaccharides arabinose, galactose and rhamnose. Fibrobacter succinogenes also degraded cell-wall pectins extensively, but at a slower rate than L. multiparus. Immunofluorescence microscopy using monoclonal antibodies revealed that, after incubation, homogalacturonans with both low and high degrees of methyl esterification were almost completely lost from walls of all cell types and from the middle lamella between cells. CONCLUSIONS: Only two of the four ruminal bacteria with pectinolytic activity degraded fresh chicory leaves, and each showed a different pattern of pectin breakdown. Degradation was greatest for F. succinogenes which also had cellulolytic activity. SIGNIFICANCE AND IMPACT OF THE STUDY: The finding of extensive removal of pectic polysaccharides from the middle lamella and the consequent decrease in particle size may explain the decreased rumination and the increased intake observed in ruminants grazing forage chicory.
Authors:
X Z Sun; K N Joblin; I G Andrew; S O Hoskin; P J Harris
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-08-18
Journal Detail:
Title:  Journal of applied microbiology     Volume:  105     ISSN:  1365-2672     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2009-01-16     Completed Date:  2010-02-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1286-97     Citation Subset:  IM    
Affiliation:
Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North, New Zealand.
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MeSH Terms
Descriptor/Qualifier:
Animals
Arabinose / metabolism
Bacteria / metabolism*
Cell Wall / metabolism*
Chicory / metabolism*
Colony Count, Microbial
Fluorescent Antibody Technique
Galactose / metabolism
Pectins / metabolism
Rhamnose / metabolism
Rumen / microbiology*
Uronic Acids / metabolism
Chemical
Reg. No./Substance:
0/Pectins; 0/Uronic Acids; 10485-94-6/Rhamnose; 147-81-9/Arabinose; 26566-61-0/Galactose; 9046-38-2/polygalacturonic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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