Document Detail

Degradation of acetylcholine in human airways: role of butyrylcholinesterase.
MedLine Citation:
PMID:  8485630     Owner:  NLM     Status:  MEDLINE    
1. Neostigmine and BW284C51 induced concentration-dependent contractions in human isolated bronchial preparations whereas tetraisopropylpyrophosphoramide (iso-OMPA) was inactive on airway resting tone. 2. Neostigmine (0.1 microM) or iso-OMPA (100 microM) increased acetylcholine sensitivity in human isolated bronchial preparations but did not alter methacholine or carbachol concentration-effect curves. 3. In the presence of iso-OMPA (10 microM) the bronchial rings were more sensitive to neostigmine. The pD2 values were, control: 6.05 +/- 0.15 and treated: 6.91 +/- 0.14. 4. Neostigmine or iso-OMPA retarded the degradation of acetylcholine when this substrate was exogenously added to human isolated airways. A marked reduction of acetylcholine degradation was observed in the presence of both inhibitors. Exogenous butyrylcholine degradation was prevented by iso-OMPA (10 microM) but not by neostigmine (0.1 microM). 5. These results suggest the presence of butyrylcholinesterase activity in human bronchial muscle and this enzyme may co-regulate the degradation of acetylcholine in this tissue.
X Norel; M Angrisani; C Labat; I Gorenne; E Dulmet; F Rossi; C Brink
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  British journal of pharmacology     Volume:  108     ISSN:  0007-1188     ISO Abbreviation:  Br. J. Pharmacol.     Publication Date:  1993 Apr 
Date Detail:
Created Date:  1993-06-04     Completed Date:  1993-06-04     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7502536     Medline TA:  Br J Pharmacol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  914-9     Citation Subset:  IM    
CNRS URA 1159, Centre Chirurgical Marie-Lannelongue, France.
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MeSH Terms
Acetylcholine / metabolism*
Acetylcholinesterase / metabolism
Benzenaminium, 4,4'-(3-oxo-1,5-pentanediyl)bis(N,N-dimethyl-N-2-propenyl-), Dibromide / pharmacology
Bronchi / enzymology,  metabolism
Butyrylcholinesterase / metabolism*
Choline / metabolism
Cholinesterase Inhibitors / pharmacology
Cholinesterase Reactivators / pharmacology
Middle Aged
Muscle Contraction / drug effects
Muscle, Smooth / drug effects
Neostigmine / pharmacology
Respiratory System / enzymology*
Tetraisopropylpyrophosphamide / pharmacology
Reg. No./Substance:
0/Cholinesterase Inhibitors; 0/Cholinesterase Reactivators; 402-40-4/Benzenaminium, 4,4'-(3-oxo-1,5-pentanediyl)bis(N,N-dimethyl-N-2-propenyl-), Dibromide; 51-84-3/Acetylcholine; 513-00-8/Tetraisopropylpyrophosphamide; 59-99-4/Neostigmine; 62-49-7/Choline; EC 3.1.1.-/Butyrylcholinesterase; EC

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