Document Detail

Degradation of 16S rRNA and attributes of viability of viable but nonculturable probiotic bacteria.
MedLine Citation:
PMID:  18444975     Owner:  NLM     Status:  MEDLINE    
AIMS: To assess the stability of 16S rRNA of viable but nonculturable (VBNC) probiotics during storage when compared with different attributes of viability. METHODS AND RESULTS: Levels of RNA of the probiotic strains Bifidobacterium longum 46, B. longum 2C and B. animalis subsp. lactis Bb-12 were monitored during storage in fermented and nonfermented foods. Cells which gradually lost their culturability in fermented products retained high level of rRNA, whereas rRNA of acid-killed control cells decreased at faster rate. Furthermore, the viability of B. longum 2C was monitored during storage by measuring changes in reductase activity, cytoplasmic membrane integrity and esterase activity using a flow cytometer. All of the culture-independent viability assays suggested that the cells remained viable during storage. In nonfermented media, the observed losses in culturability were smaller, and the changes in cell counts were comparable with the changes in rRNA levels. CONCLUSIONS: Viable but nonculturable probiotics maintain high levels of rRNA and retain properties of viable bacteria including reductase activity. Quantification of 16S rRNA complements culture-independent viability assays. SIGNIFICANCE AND IMPACT OF THE STUDY: Culture-independent viability assays allow the detection of VBNC probiotics, and can be used parallel to conventional culture-dependent methods to obtain accurate information on probiotic viability.
S J Lahtinen; H Ahokoski; J P Reinikainen; M Gueimonde; J Nurmi; A C Ouwehand; S J Salminen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-04-28
Journal Detail:
Title:  Letters in applied microbiology     Volume:  46     ISSN:  1472-765X     ISO Abbreviation:  Lett. Appl. Microbiol.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-20     Completed Date:  2008-06-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8510094     Medline TA:  Lett Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  693-8     Citation Subset:  IM    
Functional Foods Forum, University of Turku, Finland.
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MeSH Terms
Bifidobacterium / cytology*,  genetics*,  growth & development
Colony Count, Microbial
Cultured Milk Products / microbiology
Food Handling
Food Microbiology
Microbial Viability*
RNA, Bacterial / metabolism
RNA, Ribosomal, 16S / metabolism*
Time Factors
Reg. No./Substance:
0/RNA, Bacterial; 0/RNA, Ribosomal, 16S

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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