Document Detail

Degradation of 14C-labeled streptococcal cell walls by egg white lysozyme and lysosomal enzymes.
MedLine Citation:
PMID:  773836     Owner:  NLM     Status:  MEDLINE    
The resistance of native and trypsin-treated [14C] glucose-labeled cell walls to degradation by lysozyme and human lysosomal enzymes was confirmed. In contrast, chemically N-acetylated cell walls undergo significant degradation by these enzymes in the pH range of 4.5 to 5.5 without prior removal of the group-specific carbohydrate. N-acetylation after removal of the group A carbohydrate by formamide extraction renders the cell walls considerably more susceptible to these enzymes than by formamaide extraction alone. It appears, therefore, that unless N-acetylation can occur in vivo, streptococcal cell walls are minimally degraded, if at all, by human peripheral blood leukocytes or lysozyme. Examination of leukocyte extracts from normal subjects and patients with post-streptococcal syndromes revealed no qualitative differences in ability to dissolve streptococcal cell walls.
H A Gallis; S E Miller; R W Wheat
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Infection and immunity     Volume:  13     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1976 May 
Date Detail:
Created Date:  1976-08-02     Completed Date:  1976-08-02     Revised Date:  2010-09-02    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1459-66     Citation Subset:  IM    
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MeSH Terms
Carbon Radioisotopes
Cell Wall / enzymology,  metabolism,  ultrastructure
Egg White*
Lysosomes / enzymology*
Muramidase / pharmacology*
Streptococcus pyogenes / enzymology*,  metabolism
Time Factors
Reg. No./Substance:
0/Carbon Radioisotopes; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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