| Degenerate recognition of MHC class I molecules with Bw4 and Bw6 motifs by a killer cell Ig-like receptor 3DL expressed by macaque NK cells. | |
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MedLine Citation:
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PMID: 23041569 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The killer cell Ig-like receptors (KIRs) expressed on the surface of NK cells recognize specific MHC class I (MHC-I) molecules and regulate NK cell activities against pathogen-infected cells and neoplasia. In HIV infection, survival is linked to host KIR and MHC-I genotypes. In the SIV macaque model, however, the role of NK cells is unclear due to the lack of information on KIR-MHC interactions. In this study, we describe, to our knowledge, the first in-depth characterization of KIR-MHC interactions in pigtailed macaques (Macaca nemestrina). Initially, we identified three distinct subsets of macaque NK cells that stained ex vivo with macaque MHC-I tetramers loaded with SIV peptides. We then cloned cDNAs corresponding to 15 distinct KIR3D alleles. One of these, KIR049-4, was an inhibitory KIR3DL that bound MHC-I tetramers and prevented activation, degranulation, and cytokine production by macaque NK cells after engagement with specific MHC-I molecules on the surface of target cells. Furthermore, KIR049-4 recognized a broad range of MHC-I molecules carrying not only the Bw4 motif, but also Bw6 and non-Bw4/Bw6 motifs. This degenerate, yet peptide-dependent, MHC reactivity differs markedly from the fine specificity of human KIRs. |
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Authors:
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Sebastien M Maloveste; Dan Chen; Emma Gostick; Julian P Vivian; Ronald J Plishka; Ranjini Iyengar; Robin L Kruthers; Alicia Buckler-White; Andrew G Brooks; Jamie Rossjohn; David A Price; Bernard A P Lafont |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-10-05 |
Journal Detail:
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Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 189 ISSN: 1550-6606 ISO Abbreviation: J. Immunol. Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-10-22 Completed Date: 2013-01-09 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 2985117R Medline TA: J Immunol Country: United States |
Other Details:
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Languages: eng Pagination: 4338-48 Citation Subset: AIM; IM |
Affiliation:
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Non-Human Primate Immunogenetics and Cellular Immunology Unit, Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Motifs
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immunology Animals Cell Line Cells, Cultured HLA-B Antigens / chemistry, metabolism Humans Killer Cells, Natural / immunology*, metabolism* Macaca nemestrina Protein Binding / immunology Receptors, KIR3DL1 / biosynthesis, genetics*, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/HLA-B Antigens; 0/HLA-Bw4 antigen; 0/HLA-Bw6 antigen; 0/Receptors, KIR3DL1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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