Document Detail

Degenerate recognition of MHC class I molecules with Bw4 and Bw6 motifs by a killer cell Ig-like receptor 3DL expressed by macaque NK cells.
MedLine Citation:
PMID:  23041569     Owner:  NLM     Status:  MEDLINE    
The killer cell Ig-like receptors (KIRs) expressed on the surface of NK cells recognize specific MHC class I (MHC-I) molecules and regulate NK cell activities against pathogen-infected cells and neoplasia. In HIV infection, survival is linked to host KIR and MHC-I genotypes. In the SIV macaque model, however, the role of NK cells is unclear due to the lack of information on KIR-MHC interactions. In this study, we describe, to our knowledge, the first in-depth characterization of KIR-MHC interactions in pigtailed macaques (Macaca nemestrina). Initially, we identified three distinct subsets of macaque NK cells that stained ex vivo with macaque MHC-I tetramers loaded with SIV peptides. We then cloned cDNAs corresponding to 15 distinct KIR3D alleles. One of these, KIR049-4, was an inhibitory KIR3DL that bound MHC-I tetramers and prevented activation, degranulation, and cytokine production by macaque NK cells after engagement with specific MHC-I molecules on the surface of target cells. Furthermore, KIR049-4 recognized a broad range of MHC-I molecules carrying not only the Bw4 motif, but also Bw6 and non-Bw4/Bw6 motifs. This degenerate, yet peptide-dependent, MHC reactivity differs markedly from the fine specificity of human KIRs.
Sebastien M Maloveste; Dan Chen; Emma Gostick; Julian P Vivian; Ronald J Plishka; Ranjini Iyengar; Robin L Kruthers; Alicia Buckler-White; Andrew G Brooks; Jamie Rossjohn; David A Price; Bernard A P Lafont
Related Documents :
18335059 - Cardiomyocyte formation by skeletal muscle-derived multi-myogenic stem cells after tran...
22969999 - Genotoxic therapy stimulates error-prone dna repair in dormant hepatocellular cancer st...
24076049 - Transforming growth factor-β signaling controls the formation and maintenance of gut-r...
24059979 - Cell-imprinted substrates direct the fate of stem cells.
24550729 - Quantitative and qualitative deficits in neonatal lung-migratory dendritic cells impact...
23757729 - Fibrocytes represent a novel mdsc subset circulating in patients with metastatic cancer.
703759 - Synergistic interaction between uv and ionizing radiation in wild-type schizosaccharomy...
15222039 - Isolation of murine hepatic lymphocytes using mechanical dissection for phenotypic and ...
16200579 - Modulation of cd28 expression with anti-tumor necrosis factor alpha therapy in rheumato...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-10-05
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  189     ISSN:  1550-6606     ISO Abbreviation:  J. Immunol.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-22     Completed Date:  2013-01-09     Revised Date:  2013-11-06    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4338-48     Citation Subset:  AIM; IM    
Non-Human Primate Immunogenetics and Cellular Immunology Unit, Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amino Acid Motifs / immunology
Cell Line
Cells, Cultured
HLA-B Antigens / chemistry,  metabolism
Killer Cells, Natural / immunology*,  metabolism*
Macaca nemestrina
Protein Binding / immunology
Receptors, KIR3DL1 / biosynthesis,  genetics*,  metabolism*
Grant Support
Reg. No./Substance:
0/HLA-B Antigens; 0/HLA-Bw4 antigen; 0/HLA-Bw6 antigen; 0/Receptors, KIR3DL1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Peroxisome proliferator-activated receptor ? B cell-specific-deficient mice have an impaired antibod...
Next Document:  C5a receptor-dependent cell activation by physiological concentrations of desarginated C5a: insights...