Document Detail


Deformations within moving kinetochores reveal different sites of active and passive force generation.
MedLine Citation:
PMID:  22722252     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Kinetochores mediate chromosome segregation at mitosis. They are thought to contain both active, force-producing and passive, frictional interfaces with microtubules whose relative locations have been unclear. We inferred mechanical deformation within single kinetochores during metaphase oscillations by measuring average separations between fluorescently labeled kinetochore subunits in living cells undergoing mitosis. Inter-subunit distances were shorter in kinetochores moving toward poles than in those moving away. Inter-subunit separation decreased abruptly when kinetochores switched to poleward movement and decreased further when pulling force increased, suggesting that active force generation during poleward movement compresses kinetochores. The data revealed an active force-generating interface within kinetochores and a separate passive frictional interface located at least 20 nanometers away poleward. Together, these interfaces allow persistent attachment with intermittent active force generation.
Authors:
Sophie Dumont; E D Salmon; Timothy J Mitchison
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-06-21
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  337     ISSN:  1095-9203     ISO Abbreviation:  Science     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-07-23     Completed Date:  2012-08-02     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  United States    
Other Details:
Languages:  eng     Pagination:  355-8     Citation Subset:  IM    
Affiliation:
Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA. sophie.dumont@ucsf.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Cycle Proteins / genetics,  metabolism
Chromosomal Proteins, Non-Histone / genetics,  metabolism
Chromosome Segregation / genetics,  physiology*
Kinetochores / chemistry,  physiology*
Mechanical Processes
Metaphase*
Motion
Nuclear Proteins / genetics,  metabolism
Potoroidae
Grant Support
ID/Acronym/Agency:
K99 GM094335/GM/NIGMS NIH HHS; K99GM094335/GM/NIGMS NIH HHS; R01 GM039565/GM/NIGMS NIH HHS; R01GM039565/GM/NIGMS NIH HHS; R37 GM024364/GM/NIGMS NIH HHS; R37 GM039565/GM/NIGMS NIH HHS; R37GM024364/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Chromosomal Proteins, Non-Histone; 0/Nuclear Proteins; 0/centromere protein C
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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