Document Detail

Definition of regulatory network elements for T cell development by perturbation analysis with PU.1 and GATA-3.
MedLine Citation:
PMID:  12027437     Owner:  NLM     Status:  MEDLINE    
PU.1 and GATA-3 are transcription factors that are required for development of T cell progenitors from the earliest stages. Neither one is a simple positive regulator for T lineage specification, however. When expressed at elevated levels at early stages of T cell development, each of these transcription factors blocks T cell development within a different, characteristic time window, with GATA-3 overexpression initially inhibiting at an earlier stage than PU.1. These perturbations are each associated with a distinct spectrum of changes in the regulation of genes needed for T cell development. Both transcription factors can interfere with expression of the Rag-1 and Rag-2 recombinases, while GATA-3 notably blocks PU.1 and IL-7Ralpha expression, and PU.1 reduces expression of HES-1 and c-Myb. A first-draft assembly of the regulatory targets of these two factors is presented as a provisional gene network. The target genes identified here provide insight into the basis of the effects of GATA-3 or PU.1 overexpression and into the regulatory changes that distinguish the developmental time windows for these effects.
Michele K Anderson; Gabriela Hernandez-Hoyos; Christopher J Dionne; Alexandra M Arias; Dan Chen; Ellen V Rothenberg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Developmental biology     Volume:  246     ISSN:  0012-1606     ISO Abbreviation:  Dev. Biol.     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-05-24     Completed Date:  2002-07-05     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  103-21     Citation Subset:  IM    
Copyright Information:
(c) 2002 Elsevier Science (USA).
Division of Biology, California Institute of Technology, Pasadena 91125, USA.
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MeSH Terms
Base Sequence
Cell Lineage / physiology*
DNA Primers
DNA-Binding Proteins / genetics,  physiology*
GATA3 Transcription Factor
Genes, Regulator*
Mice, Knockout
Proto-Oncogene Proteins / genetics,  physiology*
RNA, Messenger / genetics
Reverse Transcriptase Polymerase Chain Reaction
T-Lymphocytes / cytology*
Trans-Activators / genetics,  physiology*
Grant Support
Reg. No./Substance:
0/DNA Primers; 0/DNA-Binding Proteins; 0/GATA3 Transcription Factor; 0/Gata3 protein, mouse; 0/Proto-Oncogene Proteins; 0/RNA, Messenger; 0/Trans-Activators; 0/proto-oncogene protein Spi-1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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