Document Detail

Defining a minimal computational unit for cerebellar long-term depression.
MedLine Citation:
PMID:  8780656     Owner:  NLM     Status:  MEDLINE    
In cerebellar long-term depression (LTD), conjunctive stimulation of parallel and climbing fiber inputs to a Purkinje neuron (PN) results in a selective depression of parallel fiber-PN synaptic strength. A similar phenomenon may be induced in the cultured PN when glutamate pulses and PN depolarization, which mimic the effects of parallel and climbing fibers, respectively, are coapplied. Here, we show that LTD can be induced in two very reduced preparations of the postsynaptic neuron; an acutely dissociated preparation and a perforated outside-out macropatch of dendritic membrane. LTD in these preparations retains properties of that seen in an intact cultured PN in that it is not induced by either glutamate pulses or depolarization alone and requires calcium influx, mGluR activation, and PKC activation for induction. As both of these preparations lack dendritic spine compartments, these findings suggest that the associative nature of LTD induction does not require this level of morphological organization.
K Narasimhan; D J Linden
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neuron     Volume:  17     ISSN:  0896-6273     ISO Abbreviation:  Neuron     Publication Date:  1996 Aug 
Date Detail:
Created Date:  1996-10-11     Completed Date:  1996-10-11     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8809320     Medline TA:  Neuron     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  333-41     Citation Subset:  IM    
Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
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MeSH Terms
Cells, Cultured / physiology
Cerebellum / cytology*
Dendrites / physiology,  ultrastructure
Long-Term Potentiation / physiology*
Membrane Potentials / physiology
Microscopy, Fluorescence
Neurons / physiology,  ultrastructure
Patch-Clamp Techniques
Grant Support

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