Document Detail


Defining the conditions for the generation of melanocytes from human embryonic stem cells.
MedLine Citation:
PMID:  16574754     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Because of their undifferentiated nature, human embryonic stem cells (hESCs) are an ideal model system for studying both normal human development and the processes that underlie disease. In the current study, we describe an efficient method for differentiating hESCs into a melanocyte population within 4-6 weeks using three growth factors: Wnt3a, endothelin-3, and stem cell factor. The hESC-derived melanocytes expressed melanocyte markers (such as microphthalmia-associated transcription factor and tyrosinase), developed melanosomes, and produced melanin. They retained the melanocyte phenotype during long-term cell culture (>90 days) and, when incorporated into human reconstructed skin, homed to the appropriate location along the basement membrane in the same manner as epidermis-derived melanocytes. They maintained a stable phenotype even after grafting of the reconstructs to immunodeficient mice. Over time in culture, the hESC-derived melanocytes lost expression of telomerase and underwent senescence. In summary, we have shown for the first time the differentiation of hESCs into melanocytes. This method provides a novel in vitro system for studying the development biology of human melanocytes.
Authors:
Dong Fang; Kim Leishear; Thiennga K Nguyen; Rena Finko; Kun Cai; Mizuho Fukunaga; Ling Li; Patricia A Brafford; Angela N Kulp; Xiaowei Xu; Keiran S M Smalley; Meenhard Herlyn
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2006-03-30
Journal Detail:
Title:  Stem cells (Dayton, Ohio)     Volume:  24     ISSN:  1066-5099     ISO Abbreviation:  Stem Cells     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-07-06     Completed Date:  2006-09-27     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9304532     Medline TA:  Stem Cells     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1668-77     Citation Subset:  IM    
Affiliation:
Program of Molecular and Cellular Oncogenesis, The Wistar Institute, Philadelphia, Pennsylvania 19104, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Culture Techniques / methods*
Cell Differentiation
Cell Lineage
Embryo, Mammalian / cytology*
Embryonic Induction*
Growth Substances / pharmacology
Humans
Melanocytes / metabolism*
Mice
Pluripotent Stem Cells / drug effects,  metabolism*
Skin / cytology
Wnt Proteins / physiology
Grant Support
ID/Acronym/Agency:
CA25874/CA/NCI NIH HHS; CA76674/CA/NCI NIH HHS; CA80999/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Growth Substances; 0/Wnt Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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