| Defining a bacteriophage T4 late promoter: absence of a "-35" region. | |
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MedLine Citation:
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PMID: 6692468 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We performed a deletion analysis to identify the minimal DNA sequence required for the function of the T4 late promoter, P23. A minipromoter derivative of P23 was constructed, containing 35 bp of T4 DNA from -18 to +17 with respect to the transcriptional initiation site. This derivative retains the TATAAATA homology and is competent to serve as a T4 late promoter both in vitro and in vivo. Its transcriptional activity in vivo is regulated identically to a wild-type plasmid-borne P23, requiring the function of T4 genes essential for late transcription, but not requiring T4 DNA replication. Recombination with the T4 phage chromosome is not significant for mini-P23 activity in vivo. |
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Authors:
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T Elliott; E P Geiduschek |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cell Volume: 36 ISSN: 0092-8674 ISO Abbreviation: Cell Publication Date: 1984 Jan |
Date Detail:
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Created Date: 1984-03-02 Completed Date: 1984-03-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0413066 Medline TA: Cell Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 211-9 Citation Subset: IM |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/J02506; J02507; K01245; K01246; K01247; K01765 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence DNA Replication DNA, Viral / genetics DNA-Directed RNA Polymerases / metabolism Genes, Viral* Mutation Operon* RNA, Viral / genetics T-Phages / genetics* Transcription, Genetic Virus Replication |
| Chemical | |
Reg. No./Substance:
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0/DNA, Viral; 0/RNA, Viral; EC 2.7.7.6/DNA-Directed RNA Polymerases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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