Document Detail

Defining antigen-specific responses with human MHC class II tetramers.
MedLine Citation:
PMID:  12170258     Owner:  NLM     Status:  MEDLINE    
Antigen-specific CD4(+) T lymphocytes play key roles in the orchestration of immune responses, including the initiation and amplification of allergic and immune-mediated disorders. Direct detection and quantitation of these cells is made possible by the use of HLA class II tetramers, soluble recombinant forms of a complex of HLA molecule and antigenic peptide that bind the antigen-specific T-cell receptor used for T-cell recognition. By using flow cytometry with fluorescent tetramers, specific T cells can be identified, recovered, and analyzed for functional markers and transcripts, helping to characterize the lineage and commitment program for individual lymphocytes. Several newly emerging uses for tetramers in clinical immunology are under development for patient management or prognosis when the number or nature of antigen-specific T cells can be clinically important. Tetramers might be useful as patient-monitoring tools for evaluating response to immunotherapy, providing a surrogate marker for the immunologic response foreshadowing a clinical response to either immune stimulation, suppression, or deviation therapeutic modalities.
Jane H Buckner; Ursula Holzer; Erik J Novak; Helena Reijonen; William W Kwok; Gerald T Nepom
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  The Journal of allergy and clinical immunology     Volume:  110     ISSN:  0091-6749     ISO Abbreviation:  J. Allergy Clin. Immunol.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-08-09     Completed Date:  2002-09-20     Revised Date:  2005-11-16    
Medline Journal Info:
Nlm Unique ID:  1275002     Medline TA:  J Allergy Clin Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  199-208     Citation Subset:  AIM; IM    
Benaroya Research Institute, Virginia Mason Research Center, 1201 Ninth Avenue, Seattle, WA 98101-2795, USA.
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MeSH Terms
Antigens / immunology*
CD4-Positive T-Lymphocytes / immunology*
Epitopes, T-Lymphocyte / immunology*
Histocompatibility Antigens Class II / immunology*
Reg. No./Substance:
0/Antigens; 0/Epitopes, T-Lymphocyte; 0/Histocompatibility Antigens Class II

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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