Document Detail


Deficient proliferation and apoptosis in the granulosa and theca interna cells of the bovine cystic follicle.
MedLine Citation:
PMID:  17615444     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of the present study was to examine the frequencies of cell proliferation and death of granulosa and theca interna layers during development of cystic follicles in order to understand the mechanisms of cystic follicle formation. Paraffin sections of cystic follicles were immunostained with antibodies against proliferating cell nuclear antigen (PCNA) and cleaved caspase-3 in order to observe proliferating and apoptotic cells, respectively. The concentrations of estradiol-17beta and progesterone in the follicular fluid of these follicles were measured by ELISA. The granulosa and theca interna layers contained both PCNA- and caspase-3-positive cells, although their numbers were limited. There was significant negative correlation between the estradiol-17beta and progesterone concentrations in the follicular fluid. Regression analysis revealed no significant correlation, except for that between the PCNA-positive cells in the theca interna and the caspase-3-positive cells in the granulosa layer. These results indicate that the granulosa and theca interna cells of the cystic follicle show weak proliferative activity and low apoptotic frequency; this implies that the cystic follicle grows slowly and then maintains a static condition without degeneration, which leads to long-term persistence of the follicle.
Authors:
Naoki Isobe; Yukinori Yoshimura
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-07-06
Journal Detail:
Title:  The Journal of reproduction and development     Volume:  53     ISSN:  0916-8818     ISO Abbreviation:  J. Reprod. Dev.     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-11-06     Completed Date:  2007-12-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9438792     Medline TA:  J Reprod Dev     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1119-24     Citation Subset:  IM    
Affiliation:
Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan. niso@hiroshima-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis*
Caspase 3 / analysis
Cattle
Cell Proliferation*
Enzyme-Linked Immunosorbent Assay
Estradiol / analysis
Female
Granulosa Cells / chemistry,  cytology*
Ovarian Follicle / chemistry,  cytology,  growth & development*
Progesterone / analysis
Proliferating Cell Nuclear Antigen / analysis
Theca Cells / chemistry,  cytology*
Chemical
Reg. No./Substance:
0/Proliferating Cell Nuclear Antigen; 50-28-2/Estradiol; 57-83-0/Progesterone; EC 3.4.22.-/Caspase 3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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