| Deficient dopamine D2 receptor function causes renal inflammation independently of high blood pressure. | |
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MedLine Citation:
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PMID: 22719934 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Renal dopamine receptors participate in the regulation of blood pressure. Genetic factors, including polymorphisms of the dopamine D(2) receptor gene (DRD2) are associated with essential hypertension, but the mechanisms of their contribution are incompletely understood. Mice lacking Drd2 (D(2)-/-) have elevated blood pressure, increased renal expression of inflammatory factors, and renal injury. We tested the hypothesis that decreased dopamine D(2) receptor (D(2)R) function increases vulnerability to renal inflammation independently of blood pressure, is an immediate cause of renal injury, and contributes to the subsequent development of hypertension. In D(2)-/- mice, treatment with apocynin normalized blood pressure and decreased oxidative stress, but did not affect the expression of inflammatory factors. In mouse RPTCs Drd2 silencing increased the expression of TNFα and MCP-1, while treatment with a D(2)R agonist abolished the angiotensin II-induced increase in TNF-α and MCP-1. In uni-nephrectomized wild-type mice, selective Drd2 silencing by subcapsular infusion of Drd2 siRNA into the remaining kidney produced the same increase in renal cytokines/chemokines that occurs after Drd2 deletion, increased the expression of markers of renal injury, and increased blood pressure. Moreover, in mice with two intact kidneys, short-term Drd2 silencing in one kidney, leaving the other kidney undisturbed, induced inflammatory factors and markers of renal injury in the treated kidney without increasing blood pressure. Our results demonstrate that the impact of decreased D(2)R function on renal inflammation is a primary effect, not necessarily associated with enhanced oxidant activity, or blood pressure; renal damage is the cause, not the result, of hypertension. Deficient renal D(2)R function may be of clinical relevance since common polymorphisms of the human DRD2 gene result in decreased D(2)R expression and function. |
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Authors:
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Yanrong Zhang; Santiago Cuevas; Laureano D Asico; Crisanto Escano; Yu Yang; Annabelle M Pascua; Xiaoyan Wang; John E Jones; David Grandy; Gilbert Eisner; Pedro A Jose; Ines Armando |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2012-06-14 |
Journal Detail:
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Title: PloS one Volume: 7 ISSN: 1932-6203 ISO Abbreviation: PLoS ONE Publication Date: 2012 |
Date Detail:
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Created Date: 2012-06-21 Completed Date: 2012-12-13 Revised Date: 2013-03-05 |
Medline Journal Info:
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Nlm Unique ID: 101285081 Medline TA: PLoS One Country: United States |
Other Details:
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Languages: eng Pagination: e38745 Citation Subset: IM |
Affiliation:
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Division of Nephrology, Department of Medicine, School of Medicine, University of Maryland, Baltimore, Maryland, United States of America. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Down-Regulation Gene Expression Profiling Hypertension / physiopathology* Immunohistochemistry Inflammation / etiology*, physiopathology Kidney Diseases / etiology*, physiopathology Mice Mice, Inbred C57BL Mice, Knockout Real-Time Polymerase Chain Reaction Receptors, Dopamine D2 / genetics, physiology* |
| Grant Support | |
ID/Acronym/Agency:
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DK039308/DK/NIDDK NIH HHS; DK090918/DK/NIDDK NIH HHS; HL023081/HL/NHLBI NIH HHS; HL068686/HL/NHLBI NIH HHS; HL074940/HL/NHLBI NIH HHS; HL092196/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Receptors, Dopamine D2 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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