|Deficiency in a very-long-chain fatty acid β-ketoacyl-coenzyme a synthase of tomato impairs microgametogenesis and causes floral organ fusion.|
|PMID: 23144186 Owner: NLM Status: MEDLINE|
|Previously, it was shown that β-ketoacyl-coenzyme A synthase ECERIFERUM6 (CER6) is necessary for the biosynthesis of very-long-chain fatty acids with chain lengths beyond C₂₈ in tomato (Solanum lycopersicum) fruits and C₂₆ in Arabidopsis (Arabidopsis thaliana) leaves and the pollen coat. CER6 loss of function in Arabidopsis resulted in conditional male sterility, since pollen coat lipids are responsible for contact-mediated pollen hydration. In tomato, on the contrary, pollen hydration does not rely on pollen coat lipids. Nevertheless, mutation in SlCER6 impairs fertility and floral morphology. Here, the contribution of SlCER6 to the sexual reproduction and flower development of tomato was addressed. Cytological analysis and cross-pollination experiments revealed that the slcer6 mutant has male sterility caused by (1) hampered pollen dispersal and (2) abnormal tapetum development. SlCER6 loss of function provokes a decrease of n- and iso-alkanes with chain lengths of C₂₇ or greater and of anteiso-alkanes with chain lengths of C₂₈ or greater in flower cuticular waxes, but it has no impact on flower cuticle ultrastructure and cutin content. Expression analysis confirmed high transcription levels of SlCER6 in the anther and the petal, preferentially in sites subject to epidermal fusion. Hence, wax deficiency was proposed to be the primary reason for the flower fusion phenomenon in tomato. The SlCER6 substrate specificity was revisited. It might be involved in elongation of not only linear but also branched very-long-chain fatty acids, leading to production of the corresponding alkanes. SlCER6 implements a function in the sexual reproduction of tomato that is different from the one in Arabidopsis: SlCER6 is essential for the regulation of timely tapetum degradation and, consequently, microgametogenesis.|
|Anna Smirnova; Jana Leide; Markus Riederer|
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|Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-11-09|
|Title: Plant physiology Volume: 161 ISSN: 1532-2548 ISO Abbreviation: Plant Physiol. Publication Date: 2013 Jan|
|Created Date: 2012-12-31 Completed Date: 2013-06-18 Revised Date: 2014-01-09|
Medline Journal Info:
|Nlm Unique ID: 0401224 Medline TA: Plant Physiol Country: United States|
|Languages: eng Pagination: 196-209 Citation Subset: IM|
|APA/MLA Format Download EndNote Download BibTex|
Cell Membrane / genetics, metabolism, physiology
Cell Wall / genetics, metabolism, physiology
Cytoplasm / genetics, metabolism
Flowers / enzymology, physiology*, ultrastructure
Gene Expression Regulation, Plant
Germ Cells, Plant / metabolism, physiology, ultrastructure
Lycopersicon esculentum / anatomy & histology, enzymology*, genetics, physiology
Membrane Lipids / metabolism
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Plant Epidermis / metabolism, ultrastructure
Plant Proteins / genetics, metabolism
Waxes / metabolism
|0/Membrane Lipids; 0/Plant Proteins; 0/Waxes; 54990-88-4/cutin; EC 188.8.131.52/3-Oxoacyl-(Acyl-Carrier-Protein) Synthase|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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