Document Detail


Deficiency in a very-long-chain fatty acid β-ketoacyl-coenzyme a synthase of tomato impairs microgametogenesis and causes floral organ fusion.
MedLine Citation:
PMID:  23144186     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Previously, it was shown that β-ketoacyl-coenzyme A synthase ECERIFERUM6 (CER6) is necessary for the biosynthesis of very-long-chain fatty acids with chain lengths beyond C₂₈ in tomato (Solanum lycopersicum) fruits and C₂₆ in Arabidopsis (Arabidopsis thaliana) leaves and the pollen coat. CER6 loss of function in Arabidopsis resulted in conditional male sterility, since pollen coat lipids are responsible for contact-mediated pollen hydration. In tomato, on the contrary, pollen hydration does not rely on pollen coat lipids. Nevertheless, mutation in SlCER6 impairs fertility and floral morphology. Here, the contribution of SlCER6 to the sexual reproduction and flower development of tomato was addressed. Cytological analysis and cross-pollination experiments revealed that the slcer6 mutant has male sterility caused by (1) hampered pollen dispersal and (2) abnormal tapetum development. SlCER6 loss of function provokes a decrease of n- and iso-alkanes with chain lengths of C₂₇ or greater and of anteiso-alkanes with chain lengths of C₂₈ or greater in flower cuticular waxes, but it has no impact on flower cuticle ultrastructure and cutin content. Expression analysis confirmed high transcription levels of SlCER6 in the anther and the petal, preferentially in sites subject to epidermal fusion. Hence, wax deficiency was proposed to be the primary reason for the flower fusion phenomenon in tomato. The SlCER6 substrate specificity was revisited. It might be involved in elongation of not only linear but also branched very-long-chain fatty acids, leading to production of the corresponding alkanes. SlCER6 implements a function in the sexual reproduction of tomato that is different from the one in Arabidopsis: SlCER6 is essential for the regulation of timely tapetum degradation and, consequently, microgametogenesis.
Authors:
Anna Smirnova; Jana Leide; Markus Riederer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-11-09
Journal Detail:
Title:  Plant physiology     Volume:  161     ISSN:  1532-2548     ISO Abbreviation:  Plant Physiol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2012-12-31     Completed Date:  2013-06-18     Revised Date:  2014-01-09    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  196-209     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
3-Oxoacyl-(Acyl-Carrier-Protein) Synthase / genetics,  metabolism*
Cell Membrane / genetics,  metabolism,  physiology
Cell Wall / genetics,  metabolism,  physiology
Cytoplasm / genetics,  metabolism
Flowers / enzymology,  physiology*,  ultrastructure
Gametogenesis, Plant*
Gene Expression Regulation, Plant
Genes, Plant*
Germ Cells, Plant / metabolism,  physiology,  ultrastructure
Lycopersicon esculentum / anatomy & histology,  enzymology*,  genetics,  physiology
Membrane Lipids / metabolism
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Phenotype
Plant Epidermis / metabolism,  ultrastructure
Plant Infertility
Plant Proteins / genetics,  metabolism
Pollination
Reproduction
Species Specificity
Substrate Specificity
Transcription, Genetic
Waxes / metabolism
Chemical
Reg. No./Substance:
0/Membrane Lipids; 0/Plant Proteins; 0/Waxes; 54990-88-4/cutin; EC 2.3.1.41/3-Oxoacyl-(Acyl-Carrier-Protein) Synthase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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