Document Detail

Deficiency of scavenger receptor class B type I negatively affects progesterone secretion in human granulosa cells.
MedLine Citation:
PMID:  20844007     Owner:  NLM     Status:  MEDLINE    
Our goal was to examine the effect of deficiency of the lipoprotein receptor, scavenger receptor class B type I (SR-BI), on progesterone secretion in human granulosa cells (HGL5). Scrambled or SR-BI small interfering RNA [knockdown (KD)] cells were exposed to dimethylsulfoxide [DMSO, vehicle for forskolin (Fo)], Fo, serum, high-density lipoprotein, low-density lipoprotein (LDL), or Fo plus lipoproteins or serum for 24 h. Progesterone secretion was lower in all of the SR-BI KD cells regardless of treatment. We examined progesterone secretion in SR-BI KD, LDL receptor KD, and double KD cells incubated with DMSO, Fo, LDL, or Fo + LDL for 6-24 h. As compared with scrambled cells, progesterone secretion was lower in SR-BI and double KD cells regardless of treatment; whereas progesterone secretion was only lower in LDL receptor KD cells incubated with LDL and Fo + LDL. We measured phosphorylation of hormone-sensitive lipase (pHSL) expression, intracellular total cholesterol (TC) mass, and progesterone secretion in scrambled and SR-BI KD cells incubated with DMSO or Fo for 2-24 h. The expression of pHSL was similar between the cells and conditions. The mean change in TC mass and progesterone secretion was lower in SR-BI KD cells exposed to DMSO and Fo. Incubating SR-BI KD cells with 22-hydroxy cholesterol did not overcome the reduction in progesterone secretion. At different time points, RNA expression of steroidogenic acute regulatory protein, side-chain cleavage, and 3β-hydroxysteroid dehydrogenase was significantly lower in SR-BI KD cells incubated with Fo. In conclusion, SR-BI protein deficiency, in part, might explain progesterone deficiency in some infertile women.
Antonina Kolmakova; Jiangxia Wang; Rebecca Brogan; Charles Chaffin; Annabelle Rodriguez
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-09-15
Journal Detail:
Title:  Endocrinology     Volume:  151     ISSN:  1945-7170     ISO Abbreviation:  Endocrinology     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-21     Completed Date:  2010-11-04     Revised Date:  2013-12-11    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5519-27     Citation Subset:  AIM; IM    
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MeSH Terms
Blotting, Western
Cell Line
Cells, Cultured
Granulosa Cells / drug effects,  metabolism,  secretion*
Lipoproteins, HDL / metabolism,  pharmacology
Lipoproteins, LDL / metabolism,  pharmacology
Progesterone / secretion*
RNA, Small Interfering
Receptors, LDL / genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Scavenger Receptors, Class B / genetics,  metabolism*
Grant Support
Reg. No./Substance:
0/Lipoproteins, HDL; 0/Lipoproteins, LDL; 0/RNA, Small Interfering; 0/Receptors, LDL; 0/SCARB1 protein, human; 0/Scavenger Receptors, Class B; 4G7DS2Q64Y/Progesterone

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