Document Detail


Deficiency of pro-apoptotic Hrk attenuates programmed cell death in the developing murine nervous system but does not affect Bcl-x deficiency-induced neuron apoptosis.
MedLine Citation:
PMID:  22043021     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The BCL-2 family includes both pro- and anti-apoptotic proteins, which regulate programmed cell death during development and in response to various apoptotic stimuli. The BH3-only subgroup of pro-apoptotic BCL-2 family members is critical for the induction of apoptotic signaling, by binding to and neutralizing anti-apoptotic BCL-2 family members. During embryonic development, the anti-apoptotic protein BCL-X(L) plays a critical role in the survival of neuronal populations by regulating the multi-BH domain protein BAX. In this study, the authors investigated the role of Harakiri (HRK), a relatively recently characterized BH3-only molecule in disrupting the BAX-BCL-X(L) interaction during nervous system development. Results indicate that HRK deficiency significantly reduces programmed cell death in the nervous system. However, HRK deficiency does not significantly attenuate the widespread apoptosis seen in the Bcl-x (-/-) embryonic nervous system, indicating that other BH3-only molecules, alone or in combination, may regulate BAX activation in immature neurons.
Authors:
Arindam P Ghosh; Jennifer D Cape; Barbara J Klocke; Kevin A Roth
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  59     ISSN:  1551-5044     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-01     Completed Date:  2011-12-19     Revised Date:  2012-01-20    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  976-83     Citation Subset:  IM    
Affiliation:
Department of Pathology, University of Alabama at Birmingham, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis*
Apoptosis Regulatory Proteins / genetics,  metabolism*
Cell Death
Gene Deletion
Mice
Mice, Inbred C57BL
Nervous System / embryology*
Neurons / cytology*
Neuropeptides / genetics,  metabolism*
bcl-2-Associated X Protein / metabolism*
bcl-X Protein / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
NS 035107/NS/NINDS NIH HHS; NS 041962/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Apoptosis Regulatory Proteins; 0/Bax protein, mouse; 0/Bid3 protein, mouse; 0/Neuropeptides; 0/bcl-2-Associated X Protein; 0/bcl-X Protein

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