Document Detail


Defibrotide mediated inhibition of serotonin, endothelin-I, thromboxane, and serum induced contraction of canine femoral and pulmonary arterial rings.
MedLine Citation:
PMID:  8914216     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Defibrotide is a polydeoxyribonucleotide derived agent with a weight average of 15 to 18 kDa. By virtue of its chemical nature, this polyelectrolyte agent exerts multiple pharmacologic actions at various plasmatic and cellular sites. In recent studies, this agent has been demonstrated to exhibit cardioprotective and vasomodulatory actions. To test the effect of defibrotide on the vascular smooth muscle contractile responses, its effects were evaluated on contractile response induced by agonists in canine femoral and pulmonary arterial smooth muscle preparation. These arterial preparations were harvested from anesthetized and anticoagulated (Heparinized 3-5 U/ml) dogs. Defibrotide was administered to dogs at 10 mg/kg, i.v. and segments of canine arteries were harvested at 30 minutes after the administration of this agent. The control and defibrotide treated canine arterial ring preparations were tested against serotonin, endothelin-I, serum and control platelet rich plasma(PRP) with arachidonic acid(AA). The contractile response of arterial rings obtained for treated groups were measured using serotonin, endothelin-I, serum and PRP/AA as agonists. The contractile action of serotonin, endothelin-I, serum and PRP/AA were inhibited by pretreatment of the animal with defibrotide. The arterial ring isolated from dogs treated with defibrotide exhibited a weaker contraction. These studies support the hypothesis that defibrotide modulates endothelial function and the response to the contractile actions of various agonists may be related to this effect.
Authors:
L H Yang; D A Hoppensteadt; O Iqbal; J Fareed
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Thrombosis research     Volume:  84     ISSN:  0049-3848     ISO Abbreviation:  Thromb. Res.     Publication Date:  1996 Nov 
Date Detail:
Created Date:  1997-02-28     Completed Date:  1997-02-28     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  0326377     Medline TA:  Thromb Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  167-77     Citation Subset:  IM    
Affiliation:
Department of Pathology, Loyola University Chicago, Stritch School of Medicine, Maywood, IL 60153, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood
Dogs
Dose-Response Relationship, Drug
Endothelin-1 / pharmacology*
Femoral Artery / drug effects,  physiology
Muscle Contraction / drug effects
Muscle, Smooth, Vascular / drug effects,  physiology*
Polydeoxyribonucleotides / pharmacology*
Pulmonary Artery / drug effects,  physiology
Serotonin / pharmacology*
Thromboxanes / pharmacology*
Chemical
Reg. No./Substance:
0/Endothelin-1; 0/Polydeoxyribonucleotides; 0/Thromboxanes; 438HCF2X0M/defibrotide; 50-67-9/Serotonin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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