Document Detail

Deferoxamine mesylate is toxic for retinal pigment epithelium cells in vitro, and its toxicity is mediated by p38.
MedLine Citation:
PMID:  20380623     Owner:  NLM     Status:  MEDLINE    
Deferoxamine mesylate is clinically used as a chelating agent but might induce retinopathy. To evaluate its effect on the retinal pigment epithelium (RPE), porcine RPE cells were stimulated with deferoxamine. Cell death was assessed with trypan blue exclusion assay. To investigate the pathway of cell death, the mitogen-activated protein kinases (MAPKs) Erk, JNK, and p38 were inhibited with U0126, SP600125, and SB203580, respectively. Their activity was determined by Western blot. Deferoxamine induces significant cell death in RPE cells, accompanied by phosphorylation of p38 and Erk. Inhibition of p38 attenuates cell death. In conclusion, deferoxamine is directly toxic on RPE cells, its toxicity depending on p38.
Alexa Klettner; Stefan Koinzer; Vicki Waetzig; Thomas Herdegen; Johann Roider
Related Documents :
9974223 - Lovastatin triggers an apoptosis-like cell death process in the fungus mucor racemosus.
9700673 - Influence of bdnf and fcs on viability and programmed cell death (pcd) of developing co...
9725553 - Enzyme kinetic reactions and fluorochrome uptake rates measured in individual cells by ...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cutaneous and ocular toxicology     Volume:  29     ISSN:  1556-9535     ISO Abbreviation:  Cutan Ocul Toxicol     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-05-05     Completed Date:  2010-07-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101266892     Medline TA:  Cutan Ocul Toxicol     Country:  England    
Other Details:
Languages:  eng     Pagination:  122-9     Citation Subset:  IM    
University of Kiel, University Medical Center, Department of Ophthalmology, Germany. <>
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Anthracenes / therapeutic use
Blotting, Western
Butadienes / therapeutic use
Cell Death / drug effects
Cells, Cultured
Deferoxamine / toxicity*
Dose-Response Relationship, Drug
Hydrogen Peroxide / toxicity
Imidazoles / therapeutic use
Iron Chelating Agents / toxicity*
Nitriles / therapeutic use
Protein Kinase Inhibitors / therapeutic use
Pyridines / therapeutic use
Retinal Pigment Epithelium / enzymology,  pathology*
Trypan Blue
p38 Mitogen-Activated Protein Kinases / antagonists & inhibitors,  physiology*
Reg. No./Substance:
0/Anthracenes; 0/Butadienes; 0/Imidazoles; 0/Iron Chelating Agents; 0/Nitriles; 0/Protein Kinase Inhibitors; 0/Pyridines; 0/SB 203580; 0/U 0126; 0/anthra(1,9-cd)pyrazol-6(2H)-one; 70-51-9/Deferoxamine; 72-57-1/Trypan Blue; 7722-84-1/Hydrogen Peroxide; EC Mitogen-Activated Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The disposition and metabolism of GW695634: A non-nucleoside reverse transcriptase inhibitor (NNRTi)...
Next Document:  Mucosal delivery of vaccines: role of mucoadhesive/biodegradable polymers.