| Defensive effects of fullerene-C60/liposome complex against UVA-induced intracellular reactive oxygen species generation and cell death in human skin keratinocytes HaCaT, associated with intracellular uptake and extracellular excretion of fullerene-C60. | |
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MedLine Citation:
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PMID: 20060738 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The UVA-irradiation of 10 J/cm(2) on HaCaT keratinocytes increased 59.1% of the intracellular reactive oxygen species (ROS) by NBT assay and the cell viability decreased to 31.5% by WST-1 assay, comparing to the non-irradiated control. In the presence of fullerene-C60 (C60) incorporated in phospholipid membrane vehicle (LiposomeFullerene: Lpsm-Flln) of 250-500 ppm, they were restored to -9.1% to +2.3% of the ROS and 83.0-84.8% of the cell viability, but scarcely restored by the liposome without C60 (Lpsm). In HaCaT cells administered with Lpsm-Flln (150 ppm), C60 was ingested at the intracellular concentrations of 1.4-21.9 ppm for 4-24 h, and, intracellular C60 was excreted by 80% at 4h after rinsing-out, and decreased to 2-10% after 24-48 h. C60 was predominantly distributed around the outside of nuclear membrane without deterioration of intact cell morphology according to fluorescent immunostain. Thus Lpsm-Flln is found to be an effective antioxidant that could preserve HaCaT keratinocytes against UVA-induced cellular injury. Lpsm-Flln has a potential to serve as a cosmetic material for skin protection against UVA. |
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Authors:
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Shinya Kato; Risa Kikuchi; Hisae Aoshima; Yasukazu Saitoh; Nobuhiko Miwa |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-12-05 |
Journal Detail:
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Title: Journal of photochemistry and photobiology. B, Biology Volume: 98 ISSN: 1873-2682 ISO Abbreviation: J. Photochem. Photobiol. B, Biol. Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-02-09 Completed Date: 2010-05-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8804966 Medline TA: J Photochem Photobiol B Country: Switzerland |
Other Details:
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Languages: eng Pagination: 144-51 Citation Subset: IM |
Copyright Information:
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Copyright 2009 Elsevier B.V. All rights reserved. |
Affiliation:
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Laboratory of Cell-Death Control BioTechnology, Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, 562, Nanatsuka, Shobara, Hiroshima 727-0023, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis* Cell Line Fullerenes / administration & dosage*, chemistry Humans Keratinocytes / metabolism, radiation effects Liposomes / chemistry* Radiation-Protective Agents / administration & dosage*, chemistry Reactive Oxygen Species / metabolism* Ultraviolet Rays* |
| Chemical | |
Reg. No./Substance:
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0/Fullerenes; 0/Liposomes; 0/Radiation-Protective Agents; 0/Reactive Oxygen Species; 99685-96-8/fullerene C60 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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