Document Detail

Defensive effects of fullerene-C60/liposome complex against UVA-induced intracellular reactive oxygen species generation and cell death in human skin keratinocytes HaCaT, associated with intracellular uptake and extracellular excretion of fullerene-C60.
MedLine Citation:
PMID:  20060738     Owner:  NLM     Status:  MEDLINE    
The UVA-irradiation of 10 J/cm(2) on HaCaT keratinocytes increased 59.1% of the intracellular reactive oxygen species (ROS) by NBT assay and the cell viability decreased to 31.5% by WST-1 assay, comparing to the non-irradiated control. In the presence of fullerene-C60 (C60) incorporated in phospholipid membrane vehicle (LiposomeFullerene: Lpsm-Flln) of 250-500 ppm, they were restored to -9.1% to +2.3% of the ROS and 83.0-84.8% of the cell viability, but scarcely restored by the liposome without C60 (Lpsm). In HaCaT cells administered with Lpsm-Flln (150 ppm), C60 was ingested at the intracellular concentrations of 1.4-21.9 ppm for 4-24 h, and, intracellular C60 was excreted by 80% at 4h after rinsing-out, and decreased to 2-10% after 24-48 h. C60 was predominantly distributed around the outside of nuclear membrane without deterioration of intact cell morphology according to fluorescent immunostain. Thus Lpsm-Flln is found to be an effective antioxidant that could preserve HaCaT keratinocytes against UVA-induced cellular injury. Lpsm-Flln has a potential to serve as a cosmetic material for skin protection against UVA.
Shinya Kato; Risa Kikuchi; Hisae Aoshima; Yasukazu Saitoh; Nobuhiko Miwa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-12-05
Journal Detail:
Title:  Journal of photochemistry and photobiology. B, Biology     Volume:  98     ISSN:  1873-2682     ISO Abbreviation:  J. Photochem. Photobiol. B, Biol.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-09     Completed Date:  2010-05-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8804966     Medline TA:  J Photochem Photobiol B     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  144-51     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Elsevier B.V. All rights reserved.
Laboratory of Cell-Death Control BioTechnology, Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, 562, Nanatsuka, Shobara, Hiroshima 727-0023, Japan.
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MeSH Terms
Cell Line
Fullerenes / administration & dosage*,  chemistry
Keratinocytes / metabolism,  radiation effects
Liposomes / chemistry*
Radiation-Protective Agents / administration & dosage*,  chemistry
Reactive Oxygen Species / metabolism*
Ultraviolet Rays*
Reg. No./Substance:
0/Fullerenes; 0/Liposomes; 0/Radiation-Protective Agents; 0/Reactive Oxygen Species; 99685-96-8/fullerene C60

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