| Defensive effects of fullerene-C60 dissolved in squalane against the 2,4-nonadienal-induced cell injury in human skin keratinocytes HaCaT and wrinkle formation in 3D-human skin tissue model. | |
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MedLine Citation:
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PMID: 20499832 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We dissolved fullerene-C60 in squalane (LipoFullerene; LF-SQ, C60-eq.: 500 ppm) and examined its defensive effects against 2,4-nonadienal (NDA)-induced cell injury in HaCaT keratinocytes and wrinkle formation in three dimensional (3D)-human skin tissue model. NDA is an analog of 4-hydroxynonenal, one of major causes for human body odor indicative of aging and a lipophilic cell injury factor. Cell viability (% of the control) decreased to 31.6% on treatment with NDA (40 microM), but it increased to 66.0-97.5% when LF-SQ of 1-4% (C60-eq.: 5-20 ppm) was administered for 5 hr before NDA addition. The defensive effect by LF-SQ was superior to that of "squalane" alone at the same doses. NDA-induced DNA-fragmentation in HaCaT cells was suppressed by LF-SQ administered for 5 hr before NDA treatment, and LF-SQ protected HaCaT cells against apoptosis-like cell death. LF-SQ did not appreciably defend against hydrogen peroxide, though LF-SQ effectively defended against tert-butylhydroperoxide, a type of the intermediate hydrophilicity-lipophilicity degree out of other reactive oxygen species. The scanning electron microscopy demonstrated that NDA caused wrinkles and abnormal scales on keratinocytes of 3D-human skin tissue model, and structural homogeneity of the interstratum was broken, any of which were, however, markedly suppressed with LF-SQ. Squalane alone exhibited defensive effect against the skin tissue injury to some extent, but which was inferior to LF-SQ. LF-SQ might effectively capture and scavenge lipid radicals generated inside the cell membrane, because squalane acts as a lipophilic carrier of C60. C60 dissolved in squalane can be expected to serve as a cosmeceutical ingredient for anti-wrinkle formation. |
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Authors:
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Shinya Kato; Hisae Aoshima; Yasukazu Saitoh; Nobuhiko Miwa |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biomedical nanotechnology Volume: 6 ISSN: 1550-7033 ISO Abbreviation: J Biomed Nanotechnol Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-05-26 Completed Date: 2010-06-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101230869 Medline TA: J Biomed Nanotechnol Country: United States |
Other Details:
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Languages: eng Pagination: 52-8 Citation Subset: IM |
Affiliation:
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Laboratory of Cell-Death Control BioTechnology, Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, 562 Nanatsuka, Shobara, Hiroshima 727-0023, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aldehydes
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toxicity* Cell Line Cell Survival / drug effects Drug Interactions Fullerenes / pharmacology* Humans In Situ Nick-End Labeling Keratinocytes / cytology, drug effects*, metabolism, pathology Models, Biological Skin Aging / drug effects*, pathology Squalene / analogs & derivatives*, pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Aldehydes; 0/Fullerenes; 111-01-3/squalane; 111-02-4/Squalene; 6750-03-4/2,4-nonadienal; 99685-96-8/fullerene C60 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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