Document Detail


Defensive conditioning-related increase in AP-1 transcription factor in the rat cortex.
MedLine Citation:
PMID:  10101233     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the studies reported herein, electrophoretic mobility shift assay (EMSA) and immunocytochemistry have been applied to document increased levels of AP-1 transcription factor, and its major component, c-Fos in the rat brain following behavioral training of two-way active avoidance. A single training session (50 trials) provoked elevation of AP-1 in the visual, sensory and limbic cortex but not in the hippocampus. A session following long term training (10 sessions, up to asymptotic level of performance) had much smaller effect on AP-1 levels in the visual cortex than single training session. The long term training was used to ensure that observed effects were related to acquisition of the reaction rather than simply to behavioral performance. Supershift EMSA analysis with antibodies directed at individual AP-1 components revealed that AP-1 extracted from the brains of trained as well as naive animals is composed of the same proteins, i.e., in order of relative level within the protein family: c-Fos, Fos B, Fra-2, and Jun D, Jun B, c-Jun. These studies reinforce the notion that transcription factors as regulators of gene expression-and AP-1 in particular-may respond to behavioral stimulation and furthermore may play a role in acquisition of behavioral reactions.
Authors:
K Lukasiuk; A Savonenko; E Nikolaev; M Rydz; L Kaczmarek
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Brain research. Molecular brain research     Volume:  67     ISSN:  0169-328X     ISO Abbreviation:  Brain Res. Mol. Brain Res.     Publication Date:  1999 Apr 
Date Detail:
Created Date:  1999-05-21     Completed Date:  1999-05-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8908640     Medline TA:  Brain Res Mol Brain Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  64-73     Citation Subset:  IM    
Copyright Information:
Copyright 1999 Elsevier Science B.V.
Affiliation:
Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology, Pasteura 3, 02-093, Warsaw, Poland.
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MeSH Terms
Descriptor/Qualifier:
Animals
Avoidance Learning / physiology*
Behavior, Animal / physiology
Conditioning (Psychology) / physiology
Cyclic AMP Response Element-Binding Protein / analysis
DNA Probes
Entorhinal Cortex / chemistry,  metabolism*
Gene Expression / physiology
Hippocampus / chemistry,  metabolism
Proto-Oncogene Proteins c-fos / analysis
Proto-Oncogene Proteins c-jun / analysis
Rats
Rats, Wistar
Somatosensory Cortex / chemistry,  metabolism*
Transcription Factor AP-1 / genetics*,  metabolism
Chemical
Reg. No./Substance:
0/Cyclic AMP Response Element-Binding Protein; 0/DNA Probes; 0/Proto-Oncogene Proteins c-fos; 0/Proto-Oncogene Proteins c-jun; 0/Transcription Factor AP-1

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