Document Detail

Defects in conidiophore development and conidium-macrophage interactions in a dioxygenase mutant of Aspergillus fumigatus.
MedLine Citation:
PMID:  18443090     Owner:  NLM     Status:  MEDLINE    
Oxygenated fatty acids, or oxylipins, play an essential role in physiological signaling and developmental processes in animals, plants, and fungi. Previous characterization of three Aspergillus fumigatus dioxygenases (PpoA, PpoB, and PpoC), similar in sequence to mammalian cyclooxygenases, showed that PpoA is responsible for the production of the oxylipins 8R-hydroperoxyoctadecadienoic acid and 5S,8R-dihydroxy-9Z,12Z-octadecadienoic acid and that PpoC is responsible for 10R-hydroxy-8E,12Z-hydroperoxyoctadecadienoic acid. Here, Delta ppo mutants were characterized to elucidate the role of fungal dioxygenases in A. fumigatus development and host interactions. The Delta ppoC strain displayed distinct phenotypes compared to those of other Delta ppo mutants and the wild type, including altered conidium size, germination, and tolerance to oxidative stress as well as increased uptake and killing by primary alveolar macrophages. These experiments implicate oxylipins in pathogen development and suggest that Delta ppoC represents a useful model for studying the A. fumigatus-host interaction.
Taylor R T Dagenais; Dawoon Chung; Steven S Giles; Christina M Hull; David Andes; Nancy P Keller
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2008-04-28
Journal Detail:
Title:  Infection and immunity     Volume:  76     ISSN:  1098-5522     ISO Abbreviation:  Infect. Immun.     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-06-20     Completed Date:  2008-07-08     Revised Date:  2014-09-10    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3214-20     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Animals, Outbred Strains
Aspergillosis / microbiology*
Aspergillus fumigatus / enzymology*,  genetics,  pathogenicity,  physiology
Dioxygenases / genetics*,  metabolism
Fungal Proteins / genetics,  metabolism
Host-Pathogen Interactions*
Macrophages, Alveolar / immunology,  microbiology
Mice, Inbred C57BL
Mice, Inbred ICR
Oxidative Stress
Spores, Fungal / physiology*
Grant Support
1 R01 AL065728-01//PHS HHS; R01 AI064287/AI/NIAID NIH HHS; R01 AI064287-05/AI/NIAID NIH HHS
Reg. No./Substance:
0/Fungal Proteins; EC 1.13.11.-/Dioxygenases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Hemolysin of uropathogenic Escherichia coli evokes extensive shedding of the uroepithelium and hemor...
Next Document:  Cholesterol depletion reduces Helicobacter pylori CagA translocation and CagA-induced responses in A...