Document Detail

Defect in UV-induced unscheduled DNA synthesis in cultured epidermal keratinocytes from xeroderma pigmentosum.
MedLine Citation:
PMID:  2432425     Owner:  NLM     Status:  MEDLINE    
DNA repair synthesis in 8 explant-outgrowth cultures of epidermal cells isolated from variant and complementation groups A and E of xeroderma pigmentosum (XP) was examined by measuring unscheduled DNA synthesis (UDS) on autoradiographs. The extents of UDS in XP epidermal cells were compared with those in normal epidermal cells obtained from 26 subjects. In both normal and XP epidermal cells, UDS was induced dose-dependently by radiation at doses of 5-20 J/m2. XP epidermal cells showed various extents of defect in DNA repair depending on the type of XP. In XP-A, the extent of UDS in epidermal cells was very low, being seen in only 3-10% of the normal epidermal cells. But epidermal cells isolated from XP-E and XP-variants exhibited relatively high levels of residual DNA repair; i.e., 69-84% of the control in XP-E and 67-85% in XP-variant. The extents of UDS in XP epidermal cells were almost the same as those in fibroblastic cells isolated from the same specimens.
S Kondo; Y Satoh; T Kuroki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Mutation research     Volume:  183     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  1987 Jan 
Date Detail:
Created Date:  1987-02-10     Completed Date:  1987-02-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  95-101     Citation Subset:  IM    
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MeSH Terms
Cells, Cultured
DNA / biosynthesis,  radiation effects*
DNA Repair*
Dose-Response Relationship, Radiation
Epidermis / cytology,  metabolism,  radiation effects*
Fibroblasts / metabolism,  radiation effects
Ultraviolet Rays*
Xeroderma Pigmentosum / genetics*
Reg. No./Substance:
68238-35-7/Keratins; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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