Document Detail


Deep-tissue photoacoustic tomography of Förster resonance energy transfer.
MedLine Citation:
PMID:  23884608     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Förster resonance energy transfer (FRET) is a distance-dependent process that transfers excited state energy from a donor molecule to an acceptor molecule without the emission of a photon. The FRET rate is determined by the proximity between the donor and the acceptor molecules; it becomes significant only when the proximity is within several nanometers. Therefore, FRET has been applied to visualize interactions and conformational changes of biomolecules, such as proteins, lipids, and nucleic acids that cannot be resolved by optical microscopy. Here, we report photoacoustic tomography of FRET efficiency at a 1-cm depth in chicken breast tissue, whereas conventional high-resolution fluorescence imaging is limited to <0.1  cm. Photoacoustic tomography is expected to facilitate the examination of FRET phenomena in living organisms.
Authors:
Yu Wang; Jun Xia; Lihong V Wang
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  18     ISSN:  1560-2281     ISO Abbreviation:  J Biomed Opt     Publication Date:  2013 Oct 
Date Detail:
Created Date:  2013-07-25     Completed Date:  2014-01-22     Revised Date:  2014-07-07    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  101316     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Chickens
Fluorescence Resonance Energy Transfer / methods*
Fluorescent Dyes / chemistry
Models, Biological
Muscle, Skeletal / chemistry
Phantoms, Imaging
Photoacoustic Techniques / methods*
Rhodamines / chemistry
Tomography / methods*
Grant Support
ID/Acronym/Agency:
DP1 EB016986/DP/NCCDPHP CDC HHS; DP1 EB016986/EB/NIBIB NIH HHS; R01 CA134539/CA/NCI NIH HHS; R01 CA157277/CA/NCI NIH HHS; R01 CA159959/CA/NCI NIH HHS; R01 CA159959/CA/NCI NIH HHS; R01 EB000712/EB/NIBIB NIH HHS; R01 EB008085/EB/NIBIB NIH HHS; U54 CA136398/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/Rhodamines; 989-38-8/rhodamine 6G

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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