| Deep sequencing identifies new and regulated microRNAs in Schmidtea mediterranea. | |
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MedLine Citation:
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PMID: 19553344 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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MicroRNAs (miRNAs) play important roles in directing the differentiation of cells down a variety of cell lineage pathways. The planarian Schmidtea mediterranea can regenerate all lost body tissue after amputation due to a population of pluripotent somatic stem cells called neoblasts, and is therefore an excellent model organism to study the roles of miRNAs in stem cell function. Here, we use a combination of deep sequencing and bioinformatics to discover 66 new miRNAs in S. mediterranea. We also identify 21 miRNAs that are specifically expressed in either sexual or asexual animals. Finally, we identified five miRNAs whose expression is sensitive to gamma-irradiation, suggesting they are expressed in neoblasts or early neoblast progeny. Together, these results increase the known repertoire of S. mediterranea miRNAs and identify numerous regulated miRNAs that may play important roles in regeneration, homeostasis, neoblast function, and reproduction. |
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Authors:
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Yi-Chien Lu; Magda Smielewska; Dasaradhi Palakodeti; Michael T Lovci; Stefan Aigner; Gene W Yeo; Brenton R Graveley |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-06-24 |
Journal Detail:
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Title: RNA (New York, N.Y.) Volume: 15 ISSN: 1469-9001 ISO Abbreviation: RNA Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-07-20 Completed Date: 2009-08-04 Revised Date: 2010-09-27 |
Medline Journal Info:
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Nlm Unique ID: 9509184 Medline TA: RNA Country: United States |
Other Details:
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Languages: eng Pagination: 1483-91 Citation Subset: IM |
Affiliation:
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Department of Genetics and Developmental Biology, University of Connecticut Stem Cell Institute, University of Connecticut Health Center, Farmington, Connecticut 06030-3301, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Gamma Rays Genome, Helminth MicroRNAs / chemistry, genetics*, radiation effects Models, Biological Molecular Sequence Data Nucleic Acid Conformation Planarians / cytology, genetics*, physiology Pluripotent Stem Cells / physiology, radiation effects RNA, Helminth / chemistry, genetics*, radiation effects Regeneration / genetics, physiology Sequence Analysis, RNA Species Specificity |
| Chemical | |
Reg. No./Substance:
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0/MicroRNAs; 0/RNA, Helminth |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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