Document Detail


Deep sequencing of HIV infected cells: insights into nascent transcription and host-directed therapy.
MedLine Citation:
PMID:  24850744     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
IMPORTANCE: In this study, we used mass sequencing to identify genes differentially expressed in CD4+ T cells during HIV-1 infection. To our surprise, we found many differentially expressed genes early after infection by analyzing both newly transcribed un-processed pre-mRNAs and fully processed mRNAs, but not by analyzing mRNAs alone, indicating a significant delay between transcription initiation and mRNA production early after HIV-1 infection. These results also show that important findings could be missed by the standard practice of analyzing mRNAs alone. We then derived the regulatory mechanisms driving the observed expression changes using integrative computational analyses. Further, we predicted drugs that could reverse the observed expression changes induced by HIV-1 infection, and showed that one of the predicted drugs indeed potently inhibited HIV-1 infection. This shows that it's possible to identify candidate drugs for host-directed therapy against HIV/AIDS using our genomics based approach.
Authors:
Xinxia Peng; Pavel Sova; Richard R Green; Matthew J Thomas; Marcus J Korth; Sean Proll; Jiabao Xu; Yanbing Cheng; Kang Yi; Li Chen; Zhiyu Peng; Jun Wang; Robert E Palermo; Michael G Katze
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-5-21
Journal Detail:
Title:  Journal of virology     Volume:  -     ISSN:  1098-5514     ISO Abbreviation:  J. Virol.     Publication Date:  2014 May 
Date Detail:
Created Date:  2014-5-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2014, American Society for Microbiology. All Rights Reserved.
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