Document Detail


Deep freezing of mouse one-cell embryos and oocytes using different cryoprotectants.
MedLine Citation:
PMID:  16726720     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The objective of this study was to compare iso-osmolar concentrations (1.5 M) of 1,2-propanediol, glycerol, dimethylsulphoxide and a combination of 1 M propanediol + 0.5M glycerol (PDGLY) as cryoprotectants for murine ovulated oocytes and one-cell embryos. A higher (P < 0.01) percentage of one-cell embryos developed to the two-cell stage when frozen-thawed with 1,2-propanediol (83%) as compared with glycerol (43%), dimethylsulfoxide (51%) or PDGLY (7%). Data recalculated on the basis of two-cell embryos/number of normal one-cell embryos after thawing indicated no differences among single cryoprotectant groups. More (P < 0.01) frozen-thawed, in-vitro fertilized oocytes developed to the two-cell stage when 1,2-propanediol (35%) was used as cryoprotectant as compared with glycerol (15%). Freezing-thawing resulted in a reduced number of two-cell embryos after oocytes were fertilized in-vitro as compared with fresh oocytes. 1,2-propanediol was a better cryoprotectant than glycerol, dimethylsulphoxide or PDGLY for deep freezing of murine oocytes or one-cell embryos.
Authors:
J J Hernandez-Ledezma; R W Wright
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Theriogenology     Volume:  32     ISSN:  0093-691X     ISO Abbreviation:  Theriogenology     Publication Date:  1989 Nov 
Date Detail:
Created Date:  2006-05-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  735-43     Citation Subset:  -    
Affiliation:
Department of Animal Sciences Washington State University Pullman, WA 99164-6332 USA.
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