| Deep UV resonant Raman spectroscopy for photodamage characterization in cells. | |
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MedLine Citation:
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PMID: 21483614 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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We employed deep UV (DUV) Raman spectroscopy for characterization of molecular photodamage in cells. 244 nm light excitation Raman spectra were measured for HeLa cells exposed to the excitation light for different durations. In the spectra obtained with the shortest exposure duration (0.25 sec at 16 µW/µm(2) irradiation), characteristic resonant Raman bands of adenine and guanine at 1483 cm(-1) and tryptophan and tyrosine at 1618 cm(-1) were clearly visible. With increasing exposure duration (up to 12.5 sec), these biomolecular Raman bands diminished, while a photoproduct Raman band at 1611 cm(-1) grew. By exponential function fitting analyses, intensities of these characteristic three bands were correlated with sample exposure duration at different intensities of excitation light. We then suggest practical excitation conditions effective for DUV Raman observation of cells without photodamage-related spectral distortion. |
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Authors:
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Yasuaki Kumamoto; Atsushi Taguchi; Nicholas Isaac Smith; Satoshi Kawata |
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Publication Detail:
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Type: Journal Article Date: 2011-03-18 |
Journal Detail:
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Title: Biomedical optics express Volume: 2 ISSN: 2156-7085 ISO Abbreviation: Biomed Opt Express Publication Date: 2011 |
Date Detail:
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Created Date: 2011-04-12 Completed Date: 2011-07-14 Revised Date: 2011-07-28 |
Medline Journal Info:
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Nlm Unique ID: 101540630 Medline TA: Biomed Opt Express Country: United States |
Other Details:
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Languages: eng Pagination: 927-36 Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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