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Deep Sequencing Analysis Reveals the Temporal Microbiota Changes Associated with the Development of Bovine Digital Dermatitis.
MedLine Citation:
PMID:  24866801     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Bovine Digital Dermatitis (DD) is a leading cause of lameness in dairy cattle throughout the world. Despite 35 years of research, the definitive etiologic agent associated with the disease process is still unknown. Previous studies have demonstrated that multiple bacterial species are associated with lesions, with spirochetes being the most reliably identified organism. This study details the deep sequencing based metagenomic evaluation of 48 staged DD biopsies collected during a 3 year longitudinal study of disease progression. Over 175 million sequences were evaluated utilizing both shotgun and 16S metagenomic techniques. Based on the shotgun sequencing results there was no evidence of a fungal or DNA viral etiology. The bacterial microbiota of biopsies progress through a systematic series of changes that correlate with the novel morphologic lesion scoring system developed as part of this project. This scoring system was validated as the microbiota of each stage was statistically different from those of other stages (p<0.001). The microbiota of control biopsies were the most diverse and became less diverse as lesions developed. Although Treponema spp. predominate the advanced lesions they are in relatively low abundance in the newly described early lesions that are associated with the initiation of the disease process. The consortia of Treponema spp. identified at the onset of disease changes considerably as the lesions progress through the morphologic stages identified. Results of this study support the hypothesis that DD is a polybacterial disease process and provide unique insights into the temporal changes in bacterial populations throughout lesion development.
Authors:
Adam C Krull; Jan K Shearer; Patrick J Gorden; Vickie L Cooper; Gregory J Phillips; Paul J Plummer
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-5-27
Journal Detail:
Title:  Infection and immunity     Volume:  -     ISSN:  1098-5522     ISO Abbreviation:  Infect. Immun.     Publication Date:  2014 May 
Date Detail:
Created Date:  2014-5-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2014, American Society for Microbiology. All Rights Reserved.
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