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Dedifferentiation of human epidermal melanocytes into melanoblasts in vitro.
MedLine Citation:
PMID:  22540983     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
  Melanoblasts (MB) are also called melanocyte (MC) precursor cells. In recent years, people have successfully cultivated human and mouse MB. Previous studies have shown that EDN3 induces cultivated bird MC to re-differentiate into double potential progenitor cells of MB. However, no study has reported whether in vitro cultivated human MC can be dedifferentiated. Our research on MC that were purified and cultivated in vitro found that adding 10 nm endothelin 1 (EDN1) (ET-1) to the MC medium without phorbol 12-myristate 13-acetate (PMA) induced a few MC to dedifferentiate and become a new type of cell. This new cell type was separated, purified, cloned and identified using multiple approaches. The results show that 88.7%, 8.69% and 2.5% of this new cell type were cells in the G(0) -G(1) , G(2) -M and S stages, respectively. The new cell type did not exhibit an apparent apoptotic peak, and its apoptotic rate was 0.09%. Stage I melanosomes were observed in the cytoplasm and were negative for the DOPA reaction. The cell surface antigen expression was positive for tyrosinase-related protein 2, negative or positive for c-kit and negative for S-100 and HMB45, showing that these cells were dedifferentiated MB of MC. Our findings provided evidence for atavism of mature human MC under certain conditions.
Authors:
Zhiguo Zhao; Cheng Jin; Keyun Ding; Xiaopeng Ge; Lllan Dai
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-24
Journal Detail:
Title:  Experimental dermatology     Volume:  -     ISSN:  1600-0625     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-4-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9301549     Medline TA:  Exp Dermatol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 John Wiley & Sons A/S.
Affiliation:
Department of Dermatology, the Affiliated of People's Hospital of Jiangsu University, Jiangsu, China Department of Dermatology, the Second People's Hospital of Wuxi, Wuxi, Jiangsu, China Department of Dermatology, Changzhou Wujin Chinese Medicine Hospital, Changzhou, Jiangsu, China Intensive Care Unit, the Affiliated of People's Hospital of Jiangsu University, Jiangsu, China.
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